Treg/Th17 Functional Disequilibrium in Chinese Uremia on Hemodialysis: A Link between Calcification and Cardiovascular Disease
Treg/Th17 Functional Disequilibrium in Chinese Uremia on Hemodialysis: A Link between Calcification and Cardiovascular Disease
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DOI：
10.3109/0886022x.2012.672155
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发表时间：
2012-01-01
期刊：
影响因子：
3
通讯作者：
Tang, Weixue
中科院分区：
文献类型：
--
作者：
Chen, Danyan;Huang, Xiaolong;Tang, Weixue
目的：探讨调节性T细胞(Treg)/辅助性T细胞（Th17）功能失衡与钙化的相关性，并探讨其对尿毒症患者血液透析（HD）后心血管疾病（CVD）预后的影响。方法：66例尿毒症患者中，36例HD后出现CVD（维持性血液透析（MHD）组1），30例无CVD （MHD组2）。选取20名健康志愿者作为正常对照组。分离外周血单个核细胞，用重组人骨形态发生蛋白-2 （rhBMP-2）处理。流式细胞术检测Treg和Th17频率。采用实时定量聚合酶链反应检测叉头/翼螺旋转录因子（Foxp3）和视黄酸受体相关孤儿受体- γ t (ror - γ t) mRNA的表达。采用酶联免疫吸附法检测白细胞介素-10和白细胞介素-17水平。结果：与对照组相比，rhBMP-2上调尿毒症患者Treg/Th17功能失衡，Treg和Th17频率、Foxp3和ror - γ t表达及细胞因子水平升高（p < 0.05）。MHD组1与MHD组2之间差异也有统计学意义（p < 0.05）。Treg/Th17功能不平衡不仅与钙化状态相关，而且与CVD一致。结论：Treg/Th17细胞功能失衡可能与钙化协同作用于HD后CVD的高发。
Aim: To investigate the correlation of the functional disequilibrium of regulatory T cells (Treg)/T-helper (Th17) cells with calcification and to explore the significance of their influence on the outcome of cardiovascular disease (CVD) in uremic patients after hemodialysis (HD). Methods: Out of 66 uremia patients, 36 patients had CVD after HD (maintenance hemodialysis (MHD) group1) and 30 patients did not have CVD (MHD group2). Twenty healthy volunteers were selected as normal control group. Peripheral blood mononuclear cells were isolated and treated with recombinant human bone morphogenetic protein-2 (rhBMP-2). Treg and Th17 frequencies were measured by flow cytometry. Forkhead/winged helix transcription factor (Foxp3) and retinoic acid receptor-related orphan receptor-gamma t (ROR-gamma t) mRNA expressions were measured by real-time quantitative polymerase chain reaction. Levels of interleukin (IL)-10 and IL-17 were detected by enzyme-linked immunosorbent assay. Results: When compared with controls, rhBMP-2 upregulates Treg/Th17 functional disequilibrium in uremia patients, displaying higher Treg and Th17 frequencies, Foxp3 and ROR-gamma t expressions, and levels of cytokines (p < 0.05). These differences were also significant between MHD group1 and group2 (p < 0.05). It was also observed that Treg/Th17 functional disequilibrium was not only correlated with a calcification state but also consistent with the CVD. Conclusion: The Treg/Th17 cell function disequilibrium might act synergistically with calcification in the high incidence of CVD after HD.