Electrophysiology tool construction.

Electrophysiology tool construction.
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DOI:
10.1002/0471142301.ns0626s62
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发表时间:
2013-01-01
影响因子:
--
通讯作者:
Ide, David
Ide, David
中科院分区:
其他
文献类型:
--
作者:
Ide, David

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该方案记录了目前广泛使用的各种研究人员的定制显微镜载物台系统的构建。该阶段的当前设计和构建是多次迭代的结果,整合了来自许多电生理学家的输入,这些电生理学家使用各种准备工作。因此,该工具是一种普遍适用的解决方案,适合各种最终用户的要求;其灵活的设计有利于快速和简单的配置,使其适用于多用户显微镜,因为个人研究人员可以重新配置载物台系统或拥有自己的易于更换的载物台板。此外,该阶段可以使用通常在小型研究机械车间中找到的设备制造,并且通过手头上的各种部件,机械师可以快速满足各种应用的新要求和/或修改。
This protocol documents the construction of a custom microscope stage system currently in widespread use by a wide variety of investigators. The current design and construction of this stage is the result of multiple iterations, integrating input from a number of electrophysiologists working with a variety of preparations. Thus, this tool is a generally applicable solution, suitable for a wide array of end-user requirements; its flexible design facilitates rapid and easy configuration, making it useful for multi-user microscopes, as individual researchers can reconfigure the stage system or have their own readily replaceable stage plates. Furthermore, the stage can be manufactured using equipment typically found in small research machine shops, and by keeping the various parts on hand, machinists can quickly satisfy new requests and/or modifications for a wide variety of applications.