The DYF-5 RCK and CDKL-1 CDKL5 kinases contribute differentially to shape distinct sensory neuron cilia morphologies in C. elegans.
The DYF-5 RCK and CDKL-1 CDKL5 kinases contribute differentially to shape distinct sensory neuron cilia morphologies in C. elegans.
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DOI：
10.17912/micropub.biology.000619
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发表时间：
2022
影响因子：
--
通讯作者：
Sengupta, Piali
中科院分区：
文献类型：
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作者：
Maurya, Ashish Kumar;Sengupta, Piali
保守的CCRK、RCK和CDKL5激酶在多种生物中调节纤毛长度。在秀丽隐杆线虫中，DYF-18 CCRK调节DYF-5 RCK来塑造简单和复杂的纤毛形态。CDKL5同源基因CDKL-1也被认为作用于DYF-18的下游，但独立于DYF-5调节简单杆状纤毛的长度。在这里，我们发现CDKL-1在复杂纤毛结构的调节中基本上是必不可少的。通过遗传上位实验，我们证实CDKL-1和DYF-5独立作用于纤毛结构。我们的研究结果表明，多种激酶通过不同的途径调节独特的纤毛超微结构。
The conserved CCRK, RCK, and CDKL5 kinases regulate cilia length in diverse organisms. In C. elegans , DYF-18 CCRK regulates DYF-5 RCK to shape both simple and complex cilia morphologies. The CDKL5 ortholog CDKL-1 has also been suggested to act downstream of DYF-18 but independently of DYF-5 to regulate lengths of simple rod-like cilia. Here we show that CDKL-1 is largely dispensable for regulation of complex cilia structures. Using genetic epistasis experiments, we confirm that CDKL-1 and DYF-5 act independently to control cilia architecture. Our results indicate that multiple kinases act via distinct pathways to regulate unique cilia ultrastructures.