Tomato analyzer-color test: A new tool for efficient digital phenotyping
Tomato analyzer-color test: A new tool for efficient digital phenotyping
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DOI：
10.21273/jashs.133.4.579
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发表时间：
2008-07-01
影响因子：
1.9
通讯作者：
Gray, Simon
中科院分区：
文献类型：
--
作者：
Darrigues, Audrey;Hall, Jack;Gray, Simon
通过图像分析测量植物特征有可能增加表型评估的客观性，提供适合定量分析的数据，并且与旨在结合表型和基因型数据的数据库兼容。我们描述了一个新的工具，它是在番茄分析仪（TA）软件应用程序中实现的，称为颜色测试（TACT）。该工具可以精确定量颜色和颜色均匀性，并允许使用颜色标准校准扫描设备。为了验证TACT方法的准确性和精密度，我们利用比色仪和扫描图像测量了番茄（Solanum lycopersicum L.）果实内部颜色。我们发现用TACT和色度计得到的L*、a*和b*值具有高度的相关性（r > 0.96）和线性。我们估计了与颜色参数相关的基因型方差，并表明用TACT测量的颜色和颜色均匀性归因于基因型的总表型方差比例显著高于从比色仪获得的估计值。当用TACT收集数据时，所有颜色和颜色均匀性性状的基因型方差几乎翻了一番。这种数字表型技术也可以应用于其他水果和蔬菜作物的颜色表征。
Measuring plant characteristics via image analysis has the potential to increase the objectivity of phenotypic evaluations, provides data amenable to quantitative analysis, and is compatible with databases that aim to combine phenotypic and genotypic data. We describe a new tool, which is implemented in the Tomato Analyzer (TA) software application, called Color Test (TACT). This tool allows for accurate quantification of color and color uniformity, and allows scanning devices to be calibrated using color standards. To test the accuracy and precision of TACT, we measured internal fruit color of tomato (Solanum lycopersicum L.) with a colorimeter and from scanned images. We show high correlations (r > 0.96) and linearity of L*, a*, and b* values obtained with TACT and the colorimeter. We estimated genotypic variances associated with color parameters and show that the proportion of total phenotypic variance attributed to genotype for color and color uniformity measured with TACT was significantly higher than estimates obtained from the colorimeter. Genotypic variance nearly doubled for all color and color uniformity traits when collecting data with TACT. This digital phenotyping technique can also be applied to the characterization of color in other fruit and vegetable crops.