TPE based electrochemiluminescence for ALP selective rapid one-step detection applied in vitro
TPE based electrochemiluminescence for ALP selective rapid one-step detection applied in vitro
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DOI：
10.1016/j.microc.2021.106041
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发表时间：
2021-05
影响因子：
4.8
通讯作者：
Sha Li;Juan Li;B. Geng;Xiaofeng Yang;Zhiling Song;Zengjun Li;Biyan Ding;Zhang Jing;
中科院分区：
文献类型：
--
作者：
Sha Li;Juan Li;B. Geng;Xiaofeng Yang;Zhiling Song;Zengjun Li;Biyan Ding;Zhang Jing;
首次研究了水溶液中碱性磷酸酶(ALP)的聚集诱导四苯基乙烯(TPE)电化学发光检测方法。以三乙胺(TEA)为共反应物，聚集诱导发射(AIE)发射体TPE-P呈现出较强的阳极聚集状态的电化学发光。在碱性磷酸酶存在下，磷酸基团通过酶裂解从原始的共轭结构中衍生出来，导致ECL信号减弱。这种新型发光材料应用于ECL体系，对碱性磷酸酶具有较高的选择性和灵敏度。在Tris-HCl缓冲溶液中的检出限为0.037.5U/L，线性范围为0.1~6.0U/L。该方法还用于人血清和细胞实验，成功地证明了其在细胞和血清碱性磷酸酶临床分析中的潜在应用。
The aggregation-induced electrochemiluminescence (ECL) of phosphate-containing tetraphenylethene (TPE) for alkaline phosphatase (ALP) activity detection in aqueous solution was investigated for the first time. By using the triethylamine (TEA) as the coreactant, the aggregation-induced emission (AIE) emittersTPE-phoexhibited a strong anode aggregation state electrochemical luminescence. In the presence of ALP, the phosphoric group was derived from the original conjugated structure by enzymatic lysis leading to a decreasing ECL signal. High selectivity and sensitivity to ALP were showed by this new luminescent material applied in the ECL system. The detection limit in Tris-HCl buffer was found to be 0.037 U/L, and the linear quantitative range was 0.1–6.0 U/L. This method was also tried to be used in the human serum and cell experiments and successfully demonstrated its potential application in clinical analysis of ALP levels in cell and serum.