Improving endothelialization on 316L stainless steel through wettability controllable coating by sol–gel technology
Improving endothelialization on 316L stainless steel through wettability controllable coating by sol–gel technology
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DOI：
10.1016/j.apsusc.2012.11.159
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发表时间：
2013-03
影响因子：
6.7
通讯作者：
Mingqi Wang;Yao Wang;Yijie Chen;H. Gu
中科院分区：
文献类型：
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作者：
Mingqi Wang;Yao Wang;Yijie Chen;H. Gu
通过表面覆盖的快速内皮化被认为是增加血管支架的血液相容性和减少平滑肌细胞（SMC）介导的再狭窄的一种方法。通过调节正硅酸乙酯（TEOS）和甲基三乙氧基硅烷（MTES）的比例，采用溶胶-凝胶法在316L不锈钢表面制备了润湿性不同、形貌相似的涂层。血管内皮细胞（EC）和SMC在这些基板上的粘附和增殖已被评估的细胞数量，细胞形态，和细胞骨架蛋白的表达。结果表明，EC和SMC对涂层表面的反应不同。在适度亲水涂层（接触角45.3°）下发现裸316 L的内皮化增强，其表现出对SMC的有效抑制，对EC的影响可忽略不计。这些结果有望为解决以平滑肌细胞增生为主的血管再狭窄奠定基础。
Rapid endothelialization by surface coverage is considered as a way to increase blood compatibility of the vascular stent and reduce smooth muscle cell (SMC) mediated restenosis. Coatings on 316L stainless steels with different wettabilities and similar topographies were obtained through sol–gel process by regulating the proportions of tetraethoxysilane (TEOS) and methyltriethoxysilane (MTES). Adhesion and proliferation of vascular endothelial cells (EC) and SMC on these substrates have been evaluated by cell numbers, cell morphology, and expression of cytoskeletal protein. Results showed that EC and SMC responded differently to the coated surfaces. Enhanced endothelialization of bare 316L was found at the moderately hydrophilic coating (contact angle 45.3°) which exhibited effective inhibition of SMC and negligible influence on EC. These results are expected to lay foundation for the solution of the vascular restenosis which was mainly derived from the hyperplasia of SMC.