Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep
Comparative immunoprophylactic efficacy of Haemonchus contortus recombinant enolase (rHcENO) and Con A purified native glycoproteins in sheep
复制标题
DOI：
10.1016/j.exppara.2015.04.016
复制
发表时间：
2015-07-01
影响因子：
2.1
通讯作者：
Raman, Muthusamy
中科院分区：
文献类型：
--
作者：
Kalyanasundaram, Aravindan;Jawahar, Shabnam;Raman, Muthusamy
捻转血矛线虫（Haemonchuscontortus）是世界上最重要的绵羊和山羊吸血线虫寄生虫。蠕虫烯醇化酶是一种多功能酶，参与糖酵解和入侵宿主组织。本研究中，重组H.在疫苗攻击试验中，评价捻转肌烯醇化酶（rHcENO）与Con A纯化的天然糖蛋白沿着在绵羊中的免疫预防功效。I组和II组试验羊分别用纯化的天然糖蛋白沿着的rHcENO和Con A免疫3次，并加入Montanide伊萨61 VG佐剂。用5000 L3阶段活性H激发动物。第三次免疫后21天的捻转幼虫。与对照动物相比，在rHcENO和Con A纯化的天然糖蛋白免疫的动物中观察到IgG滴度的显著增加。Con A纯化的天然糖蛋白的免疫保护效力相对高于rHcENO抗原。(C)2015 Elsevier Inc. All rights reserved.
Haemonchus contortus is the most economically important blood feeding nematode parasite of sheep and goats all over the world. Enolase in helminth parasites is a multi-functional enzyme which involves in glycolysis and host tissue invasion. In this study, the recombinant H. contortus enolase (rHcENO) was evaluated for its immunoprophylactic efficacy in sheep along with Con A purified native glycoproteins in a vaccine challenge trial. Group I and Group II experimental sheep were immunized thrice with rHcENO and Con A purified native glycoproteins along with Montanide ISA 61 VG adjuvant. The animals were challenged with 5000 L3 stage active H. contortus larvae after 21 days of third immunization. A significant increase in the IgG titre was observed in rHcENO and Con A purified native glycoproteins immunized animals as compared to the control animals. Immunoprotective efficacy of Con A purified native glycoproteins was comparatively higher than rHcENO antigen. (C) 2015 Elsevier Inc. All rights reserved.