Implementation of a multi-institutional diffuse intrinsic pontine glioma autopsy protocol and characterization of a primary cell culture
Implementation of a multi-institutional diffuse intrinsic pontine glioma autopsy protocol and characterization of a primary cell culture
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DOI：
10.1111/j.1365-2990.2012.01294.x
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发表时间：
2013-06-01
影响因子：
5
通讯作者：
Kaspers, G. J. L.
中科院分区：
文献类型：
--
作者：
Caretti, V.;Jansen, M. H. A.;Kaspers, G. J. L.
V. Caretti、M. H. A. Jansen、D. G. van Vuurden、T. Lagerweij、M. Bugiani、I. Horsman、H. Wessels、P. van der Valk、J. Cloos、D. P. Noske、W. P. Vandertop、P. Wesseling、T. Wurdinger、E. Hulleman 和 G. J. L. Kaspers (2013) 神经病理学和应用神经生物学39, 426436 多机构弥漫性内质性脑桥胶质瘤尸检方案的实施和原代细胞培养物的表征 目的：弥漫性内质性桥脑胶质瘤 (DIPG) 是一种致命的儿科恶性肿瘤。如果没有严重的发病率，肿瘤切除是不可能的，并且很少进行活检。由于缺乏主要 DIPG 材料，临床前研究实际上变得不可能，并阻碍了针对该疾病的新疗法的开发。当前研究的目的是通过制定多机构尸检方案来解决主要 DIPG 材料和临床前模型缺乏的问题。方法：在荷兰实施了尸检方案，以便在短暂的尸检间隔内获取肿瘤材料。由神经病理学家和研究人员组成的团队随时可以进行尸检并处理收获的材料。进行全脑尸检，并从所有受影响的脑区收集主要 DIPG 材料和健康组织。最后，该研究还包括家长的系统评估。结果：进行了五次尸检。死亡与尸检之间的平均时间间隔为 3 小时（范围 24）。所有肿瘤均被分级为胶质母细胞瘤。没有一位家长后悔自己的参与选择，他们都因捐献孩子的组织以帮助未来的 DIPG 患者而感到安慰。此外，我们还利用死后材料开发并表征了第一个 DIPG 细胞培养物。结论：在这里，我们表明，为研究目的获取死后 DIPG 肿瘤组织是可行的，且延迟时间短，尸检程序令参与家长满意，并且适合临床前 DIPG 模型的开发。
V. Caretti, M. H. A. Jansen, D. G. van Vuurden, T. Lagerweij, M. Bugiani, I. Horsman, H. Wessels, P. van der Valk, J. Cloos, D. P. Noske, W. P. Vandertop, P. Wesseling, T. Wurdinger, E. Hulleman and G. J. L. Kaspers (2013) Neuropathology and Applied Neurobiology39, 426436 Implementation of a multi-institutional diffuse intrinsic pontine glioma autopsy protocol and characterization of a primary cell culture Aims: Diffuse intrinsic pontine glioma (DIPG) is a fatal paediatric malignancy. Tumour resection is not possible without serious morbidity and biopsies are rarely performed. The resulting lack of primary DIPG material has made preclinical research practically impossible and has hindered the development of new therapies for this disease. The aim of the current study was to address the lack of primary DIPG material and preclinical models by developing a multi-institutional autopsy protocol. Methods: An autopsy protocol was implemented in the Netherlands to obtain tumour material within a brief post mortem interval. A team of neuropathologists and researchers was available at any time to perform the autopsy and process the material harvested. Whole brain autopsy was performed and primary DIPG material and healthy tissue were collected from all affected brain areas. Finally, the study included systematic evaluation by parents. Results: Five autopsies were performed. The mean time interval between death and time of autopsy was 3h (range 24). All tumours were graded as glioblastoma. None of the parents regretted their choice to participate, and they all derived comfort in donating tissue of their child in the hope to help future DIPG patients. In addition, we developed and characterized one of the first DIPG cell cultures from post mortem material. Conclusion: Here we show that obtaining post mortem DIPG tumour tissue for research purposes is feasible with short delay, and that the autopsy procedure is satisfying for participating parents and can be suitable for the development of preclinical DIPG models.