Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein.
Regulation of cell polarity determinants by the Retinoblastoma tumor suppressor protein.
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DOI：
10.1038/srep22879
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发表时间：
2016-03-14
影响因子：
4.6
通讯作者：
Arnosti DN
中科院分区：
文献类型：
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作者：
Payankaulam S;Yeung K;McNeill H;Henry RW;Arnosti DN
除了它们在细胞周期中的典型作用之外，RB家族蛋白还调节许多发育途径，尽管其机制仍然不清楚。我们发现，果蝇Rbf 1与高度保守的顶端-基底和平面细胞极性通路的编码基因的组成部分，这表明可能的调节作用。在这里，我们表明果蝇组织中Rbf 1的缺失确实与翅膀和眼睛的极性缺陷有关。关键极性基因aPKC、par 6、旺、pk和fmi上调，aPKC突变抑制Rbf 1诱导的表型。RB对细胞极性的控制可能是一种进化上保守的功能，在癌症转移中具有重要意义。
In addition to their canonical roles in the cell cycle, RB family proteins regulate numerous developmental pathways, although the mechanisms remain obscure. We found that Drosophila Rbf1 associates with genes encoding components of the highly conserved apical–basal and planar cell polarity pathways, suggesting a possible regulatory role. Here, we show that depletion of Rbf1 in Drosophila tissues is indeed associated with polarity defects in the wing and eye. Key polarity genes aPKC, par6, vang, pk, and fmi are upregulated, and an aPKC mutation suppresses the Rbf1-induced phenotypes. RB control of cell polarity may be an evolutionarily conserved function, with important implications in cancer metastasis.