Identification of SCARA5 as a Potential Biomarker for Oral Squamous Cell Carcinoma using MALDI-TOF-MS Analysis
Identification of SCARA5 as a Potential Biomarker for Oral Squamous Cell Carcinoma using MALDI-TOF-MS Analysis
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使用 MALDI-TOF-MS 分析鉴定 SCARA5 作为口腔鳞状细胞癌的潜在生物标志物
DOI：
10.1002/prca.201700180
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发表时间：
2018-09-01
影响因子：
2
通讯作者：
Zhong, Lai-ping
中科院分区：
文献类型：
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作者：
Liu, Ying;Tan, Yi-ran;Zhong, Lai-ping
目的寻找口腔鳞状细胞癌（oral and squamous cell carcinoma，OSCC）诊断模型中的潜在生物标志物，并进一步验证该生物标志物的有效性。另外，采用免疫组化、PCR、ELISA和Western blot技术检测scara5在临床标本和细胞系中的表达。此外，scara5表达下调与细胞增殖和侵袭有关。血清Scara5检测可作为口腔鳞癌早期诊断的血清标志物。该研究的临床相关性在于通过蛋白质组学找到生物标志物，并随后用临床样品和细胞系对其进行验证。
PurposeTo find the potential biomarkers in the diagnostic model of oral and squamous cell carcinoma (OSCC), and to further validate the biomarker.Experimental designWith the MALDI-TOF-MS analysis between tissues from oral cancer patients and normal oral mucosa from healthy controls, scavenger receptor class A member 5 (scara5) is found to be potentially significant after searching the protein database. In addition, Immunohistochemical staining, PCR, ELISA, and Western blot technique are used to detect scara5 expression in clinical samples and cell lines.ResultsIn this study, the results indicate that scara5 expression is decreased in tumor group in the MALDI-TOF-MS analysis. Furthermore, down-regulation of scara5 expression is related with cell proliferation and invasion. Serum scara5 detection can discriminate OSCC samples from normal samples with high sensitivity.Conclusions and clinical relevanceScara5 has the potential to be considered as a serum biomarker in the early diagnosis of OSCC. The clinical relevance of the study lies in finding the biomarker by proteomics and subsequently validating it with clinical samples and cell lines.