Dendritically released peptides act as retrograde modulators of afferent excitation in the supraoptic nucleus in vitro
Dendritically released peptides act as retrograde modulators of afferent excitation in the supraoptic nucleus in vitro
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DOI：
10.1016/s0896-6273(00)80971-x
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发表时间：
1997-10-01
期刊：
影响因子：
16.2
通讯作者：
Pittman, QJ
中科院分区：
文献类型：
--
作者：
Kombian, SB;Mouginot, D;Pittman, QJ
已知催产素（OXT）和加压素（VP）从大细胞神经元的树突释放。在这里，我们表明，这些肽减少诱发的突触前机制，肽拮抗剂阻断和模仿内源性肽酶抑制的效果。树突状释放的肽，引起与去极化实现高频刺激的传入或电流注入到一个单独的神经元，诱导短期突触抑制类似的外源性肽应用程序，并防止肽拮抗剂。因此，树突状释放的肽通过激活突触前OXT和/或VP受体来抑制大细胞神经元中诱发的EPSC。这种对传入兴奋的逆行调节作用可能作为一种反馈机制，允许肽能神经分泌神经元自动调节自己的活动。
Oxytocin (OXT) and vasopressin (VP) are known to be released from dendrites of magnocellular neurons. Here, we show that these peptides reduced evoked EPSCs by a presynaptic mechanism, an effect blocked by peptide antagonists and mimicked by inhibition of endogenous peptidases. Dendritic release of peptides, elicited with depolarization achieved by high frequency stimulation of afferents or with current injection into an individual neuron, induced short-term synaptic depression similar to that seen following exogenous peptide application and was prevented by peptide antagonists. Thus, dendritically released peptides depress evoked EPSCs in magnocellular neurons by activating presynaptic OXT and/or VP receptors. Such a retrograde modulatory action on afferent excitation may serve as a feedback mechanism to permit peptidergic neurosecretory neurons to autoregulate their own activity.