Interactions among Delta, Serrate and Fringe modulate Notch activity during Drosophila wing development.
Interactions among Delta, Serrate and Fringe modulate Notch activity during Drosophila wing development.
复制标题
作者：
T. Klein;A. M. Arias
Notch信号通路在翅原基的发育过程中起着重要的作用，特别是在翅叶和翅缘的发育过程中。在这些过程中，Notch的活性由背特异性核蛋白Apterous的活性控制，该蛋白调节Notch配体、Serrate和Fringe信号分子的表达。另一个Notch配体Delta也在翅膀的发育和图案化中发挥作用。有人提出，条纹调制锯齿和三角洲的能力，通过刻槽信号，从而限制刻槽信号的背腹侧边界的发展机翼叶片。在这里，我们报告的结果，旨在建立在机翼发展的边缘，锯齿和三角洲之间的关系的实验。我们发现，锯齿是不需要的启动翅膀的发展，而是为扩展和早期图案的翅膀原基。我们提供的证据表明，在开始的翅膀发展，三角洲的无翅的控制下，可能是Notch配体在这个过程中。另外，我们发现Fringe函数需要Su（H）.我们的研究结果表明，Notch信号在翅膀发育过程中依赖于Notch配体之间的积极和显性负相互作用之间的仔细平衡，其中一些是由边缘介导的。
The Notch signalling pathway plays an important role during the development of the wing primordium, especially of the wing blade and margin. In these processes, the activity of Notch is controlled by the activity of the dorsal specific nuclear protein Apterous, which regulates the expression of the Notch ligand, Serrate, and the Fringe signalling molecule. The other Notch ligand, Delta, also plays a role in the development and patterning of the wing. It has been proposed that Fringe modulates the ability of Serrate and Delta to signal through Notch and thereby restricts Notch signalling to the dorsoventral boundary of the developing wing blade. Here we report the results of experiments aimed at establishing the relationships between Fringe, Serrate and Delta during wing development. We find that Serrate is not required for the initiation of wing development but rather for the expansion and early patterning of the wing primordium. We provide evidence that, at the onset of wing development, Delta is under the control of apterous and might be the Notch ligand in this process. In addition, we find that Fringe function requires Su(H). Our results suggest that Notch signalling during wing development relies on careful balances between positive and dominant negative interactions between Notch ligands, some of which are mediated by Fringe.