Production of phloroglucinol by Escherichia coli using a stationary-phase promoter
Production of phloroglucinol by Escherichia coli using a stationary-phase promoter
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大肠杆菌使用稳定期启动子生产间苯三酚
DOI：
10.1007/s10529-011-0638-0
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Xian, Mo
中科院分区：
文献类型：
--
作者：
Cao, Yujin;Xian, Mo
利用一种新的宿主-载体系统对大肠杆菌进行代谢工程改造，以生产间苯三酚。将荧光假单胞菌（Pseudomonasfluorescens）生物合成的关键基因phlD（编码III型聚酮合酶）在大肠杆菌中表达。大肠杆菌中使用fic基因的静止期启动子和高拷贝质粒。在摇瓶中，该工程菌产间苯三酚最高达0.28g/L，产率为0.014g/L·h。20 h后约9.2%的葡萄糖转化为间苯三酚。与目前广泛使用的T7启动子系统相比，该菌株不需要IPTG诱导，间苯三酚的最终效价提高了22%。
Escherichia coli was metabolically engineered using a new host-vector system to produce phloroglucinol. The key biosynthetic gene phlD (encoding a type III polyketide synthase) from Pseudomonas fluorescens was expressed in E. coli using the stationary-phase promoter of the fic gene and a high-copy plasmid. In shake-flasks, the engineered strain produced phloroglucinol up to 0.28 g/l with a productivity of 0.014 g/l h. About 9.2% of the glucose consumed was converted to phloroglucinol after 20 h. Compared with the widely used inducible T7 promoter system, this strain did not require IPTG induction and the final titer of phloroglucinol was 22% higher.