Increased interleukin-4, interleukin-5, and interferon-γ in airway CD4+ and CD8+ T cells in atopic asthma
Increased interleukin-4, interleukin-5, and interferon-γ in airway CD4+ and CD8+ T cells in atopic asthma
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DOI：
10.1164/rccm.200310-1416oc
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发表时间：
2005-02-01
影响因子：
24.7
通讯作者：
Johnston, SL
中科院分区：
文献类型：
--
作者：
Cho, SH;Stanciu, LA;Johnston, SL
Th 2细胞因子在哮喘中的产生增加已被广泛接受，但哮喘患者气道CD 4（+）T细胞的过度产生尚未得到证实，也与疾病严重程度无关。气道CD 8（+）T细胞1型和2型细胞因子的产生在哮喘中的重要性尚不清楚。我们研究了正常受试者和哮喘受试者的IFN-γ、白细胞介素（IL）-4和IL-5产生的CD 4（+）和CD 8（+）血液和痰液T细胞的频率，并比较了细胞亚群、受试者组和体外刺激和无体外刺激的身体隔室，并研究了细胞因子产生与哮喘严重程度之间的关系。哮喘患者痰液中未受刺激的CD 4（+）和CD 8（+）T细胞产生的IL-4、IL-5和IFN-γ增加，并与疾病严重程度相关，CD 8（+）T细胞比CD 4（+）T细胞更多。痰液中CD 8（+）T细胞产生1型和2型细胞因子的频率与CD 4（+）T细胞相似。体外刺激使外周血细胞因子的产生偏向IFN-γ的产生，哮喘受试者比正常受试者显著增加。这些数据表明痰液中CD 4（+）和CD 8（+）T细胞中1型和2型细胞因子的产生增加，并将产生与疾病严重程度相关。血液中的发现并不反映气道中的发现。
Increased Th2 cytokine production in asthma is widely accepted, but excess production by asthmatic human airway CD4(+) T cells has not been demonstrated, nor has a relationship with disease severity. The importance of airway CD8(+) T cell type 1 and type 2 cytokine production in asthma is unknown. We investigated frequencies of IFN-gamma, interleukin (IL)-4 and IL-5 producing CD4(+) and CD8(+) blood and sputum T cells from normal subjects and subjects with asthma and compared between cell subsets, subject groups, and body compartments with and without in vitro stimulation and investigated relationships between cytokine production and asthma severity. Production of IL-4, IL-5, and IFN-gamma by unstimulated sputum CD4(+) and CD8(+) T cells was increased in subjects with asthma and related to disease severity, more for CD8(+) than for CD4(+) T cells. Frequencies of sputum CD8(+) T cells producing type 1 and type 2 cytokines were similar to those of CD4(+) T cells. In vitro stimulation polarized peripheral blood cytokine production toward IFN-gamma production, significantly more in subjects with asthma than in normal subjects. These data demonstrate increased type 1 and 2 cytokine production in CD4(+) and CD8(+) T cells in sputum and relate production to disease severity. Findings in blood did not reflect those in airways.