Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA
Enzymatic approaches and bisulfite sequencing cannot distinguish between 5-methylcytosine and 5-hydroxymethylcytosine in DNA
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DOI：
10.2144/000113403
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发表时间：
2010-04-01
期刊：
影响因子：
2.7
通讯作者：
Dunican, Donncha S.
中科院分区：
文献类型：
--
作者：
Nestor, Colm;Ruzov, Alexey;Dunican, Donncha S.
DNA胞嘧啶甲基化（5 mC）在哺乳动物细胞中高度丰富，并且与转录抑制相关。最近，在某些人类细胞类型中检测到高水平的羟甲基胞嘧啶（hmC）;然而，其作用尚不清楚。由于5 mC和hmC之间的结构相似性，尚不清楚5 mC分析是否可以区分这些核苷酸。在这里，我们表明，5 mC和hmC是实验上无法区分使用已建立的5 mC映射方法，从而意味着现有的5 mC数据集将需要仔细重新评估的背景下，可能存在的hmC。使用5 mC单克隆抗体可促进5 mC和hmC DNA序列的潜在差异富集。
DNA cytosine methylation (5mC) is highly abundant in mammalian cells and is associated with transcriptional repression. Recently, hydroxymethylcytosine (hmC) has been detected at high levels in certain human cell types; however, its roles are unknown. Due to the structural similarity between 5mC and hmC, it is unclear whether 5mC analyses can discriminate between these nucleotides. Here we show that 5mC and hmC are experimentally indistinguishable using established 5mC mapping methods, thereby implying that existing 5mC data sets will require careful re-evaluation in the context of the possible presence of hmC. Potential differential enrichment of 5mC and hmC DNA sequences may be facilitated using a 5mC monoclonal antibody.