Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations
Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations
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DOI：
10.1007/s10384-010-0903-0
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发表时间：
2011-01-01
影响因子：
2.4
通讯作者：
Hayashi, Atsushi
中科院分区：
文献类型：
--
作者：
Kitagawa, Kiyotaka;Okabe, Motonori;Hayashi, Atsushi
报道超干交联羊膜（HDCL）与组织胶联合贴敷治疗角膜穿孔的初步效果。本研究纳入了3例角膜穿孔直径达3 mm的患者的3只眼。使用HDCL-AM单层贴片和组织粘合剂（2-辛基-氰基丙烯酸酯）对其进行治疗。在体外消化试验中，我们还评估了HDCL-AM对胶原酶的抵抗力。在所有三个病例中，角膜穿孔在28天内（范围，17-28天）被修复。随访3-6个月，无复发。在胶原酶消化试验中，HDCL-AM不溶解，直到48小时，而冷冻保存的AM完全溶解在60分钟内。3例角膜穿孔成功地管理使用HDCL-AM修补与组织粘合剂。在体外消化试验中，HDCL-AM对胶原酶具有抗性。HDCL-AM是一种有效的角膜穿孔基质。这种简单的手术技术可能是角膜穿孔的最初治疗选择之一。
To report the use of hyperdried cross-linked (HDCL) amniotic membrane (AM) patching with tissue adhesive as an initial therapy for corneal perforations.Cryopreserved AM was cross-linked with 0.1% glutaraldehyde and then dried using far-infrared rays and microwaves (hyperdry method). Three eyes of three patients with corneal perforations of up to 3 mm in diameter were included in this study. They were treated with a single-layer patch of HDCL-AM applied with a tissue adhesive (2-octyl-cyanoacrylate). We also evaluated the resistance of HDCL-AM to collagenases during in vitro digestion testing.In all three cases, the corneal perforations were repaired within 28 days (range, 17-28 days). No recurrence occurred during the follow-up period (3-6 months). In the collagenase digestion testing, the HDCL-AM did not dissolve until 48 h, whereas the cryopreserved AM completely dissolved within 60 min.Three cases of corneal perforations were successfully managed using HDCL-AM patching with tissue adhesive. The HDCL-AM was resistant to collagenases during in vitro digestion testing. The HDCL-AM was a useful substrate for corneal perforations. This simple surgical technique may be one of the initial therapeutic options for corneal perforations.