Biological and physico-chemical assessment of hydroxyapatite (HA) with different porosity
Biological and physico-chemical assessment of hydroxyapatite (HA) with different porosity
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DOI：
10.1016/j.bioeng.2007.08.015
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发表时间：
2007-11-01
期刊：
影响因子：
--
通讯作者：
Hildebrand, H. F.
中科院分区：
文献类型：
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作者：
Hornez, J. -C.;Chai, F.;Hildebrand, H. F.
将具有特定内部孔隙率的透明质酸（HA）装入不同的抗生素（ATBs），并对其微生物效果进行了测试。用x射线衍射、红外光谱和拉曼光谱对HA的纯度进行了控制。改变烧结温度和/或添加石墨和PMMA作为致孔剂可获得微孔和介孔。生物学试验涉及细胞活力、增殖和形态（SEM），以及肌动蛋白和血管蛋白的细胞化学染色。微孔和中孔HA样品的内部孔径分别为1 ~ 10 μ m和10 ~ 50 μ m。x射线衍射和红外光谱证实了HA的高纯度。L132细胞的细胞活力实验证实了透明质酸、石墨粉和ATB万古霉素具有良好的细胞相容性。用MC3T3-E1成骨细胞检测细胞增殖率。所有HA样品的增殖均高于对照组；微孔透明质酸诱导细胞生长最快。ATB浸渍的HA对细胞增殖也有刺激作用，但刺激程度较低。成骨细胞的细胞化学染色显示发育良好的细胞骨架和强应力纤维。用抗毒蛋白标记黏附接触灶显示细胞在不同透明质酸基质上的黏附过程较不发达。通过改变烧结温度和/或添加多孔剂，可以获得具有不同孔隙度但可控的高纯度羟基磷灰石。这种纯度和微孔隙度显著刺激细胞生长。(C) 2007 Elsevier B.V.版权所有
HA with specific internal porosities was loaded with different antibiotics (ATBs) and then tested on its microbiological effectiveness. The HA purity was controlled with X-ray diffraction, IR and Raman spectrometry. Varying the sintering temperature and/or adding graphite and PMMA as porogenous agents lead to obtained micro- and meso-porosities. The biological tests concerned cell viability, proliferation and morphology (SEM), and the cytochemical staining of actin and vinculin. The micro- and meso-porous HA samples had an internal pore size of 1-10 mu m and 10-50 mu m, respectively. X-ray diffraction and FTIR confirmed the high purity of the HA. The cell viability tests with L132 cells confirmed the excellent cytocompatibility of HA, the graphite powder and the ATB vancomycine. Proliferation rate was assessed with MC3T3-E1 osteoblasts. All HA samples produced a higher proliferation than the controls; the micro-porous HA inducing the highest cell growth. The ATB impregnated HA also stimulated cell proliferation but in lower extend. Cytochemical staining of osteoblasts revealed a well-developed cytoskeleton with strong stress fibres. Labelling of the focal adhesion contacts with anti-vinculin showed a less developed adhesion process in the cells on the different HA substrates. It was possible to realize a highly pure hydroxyapatite with different but controlled porosities by varying the sintering temperature and/or addition of a porogenous agents. This purity and the micro-porosity stimulate significantly cell growth. (C) 2007 Elsevier B.V. All rights reserved.