Retinoic acid receptors and muscle b-HLH proteins:: partners in retinoid-induced myogenesis
Retinoic acid receptors and muscle b-HLH proteins:: partners in retinoid-induced myogenesis
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DOI：
10.1038/sj.onc.1201894
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发表时间：
1998-07-02
期刊：
影响因子：
8
通讯作者：
Bonnieu, A
中科院分区：
文献类型：
--
作者：
Froeschlé, A;Alric, S;Bonnieu, A
本文报道的结果表明，视黄酸 (RA) 仅在表达 MyoD 的肌肉细胞中诱导生长停滞和分化。瞬时转染测定揭示了 MyoD（一种关键的生肌调节剂）和 RA 受体（RA 作用的主要介质）之间的功能相互作用。有趣的是，我们证明了包含 RXR-MyoD 的复合物在肌肉细胞中特定的 MyoD DNA 结合位点处被募集。此外，我们还证明了 RA 受体和肌肉碱性螺旋-环-螺旋 (b-HLH) 蛋白相互作用 身体上。突变分析表明，这种相互作用通过肌肉 b-HLH 蛋白的基本区域和 RA 受体的 DIVA 结合域发生，并且对于这两个转录因子家族之间的功能相互作用非常重要。总之，这些结果强调了影响细胞生长和分化的两组不同调节蛋白之间的新相互作用。
The results reported here indicate that retinoic acid (RA) induces growth arrest and differentiation only in MyoD-expressing muscle cells. Transient transfection assays reveal a functional interaction between MyoD, a key myogenic regulator and RA-receptors, principal mediators of RA actions, Interestingly, we demonstrate that RXR-MyoD-containing complexes are recruited at specific MyoD DNA-binding sites in muscle cells, Furthermore, we also demonstrate that RA-receptors and the muscle basic helix-loop-helix (b-HLH) proteins interact physically. Mutational analysis suggests that this interaction occurs via the basic region of muscle b-HLH proteins and the DIVA-binding domain of RA-receptors and is important for functional interactions between these two families of transcription factors. In conclusion, these results highlight novel interactions between two distinct groups of regulatory proteins that influence cell growth and differentiation.