Evidence for CD8(+) antiviral activity in cats infected with feline immunodeficiency virus
Evidence for CD8(+) antiviral activity in cats infected with feline immunodeficiency virus
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DOI：
10.1128/jvi.70.4.2474-2480.1996
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发表时间：
1996-04-01
影响因子：
5.4
通讯作者：
Tompkins, WAF
中科院分区：
文献类型：
--
作者：
Jeng, CR;English, RV;Tompkins, WAF
人类免疫缺陷病毒（HIV）引起长期无症状感染，其特征是循环CD8（+）细胞数量正常至升高，CD4（+）细胞进行性下降。据推测，由CD8（+）T细胞驱动的HIV特异性抗病毒活性可能在此期间控制病毒复制，并维持疾病的临床无症状阶段。由猫免疫缺陷病毒（FIV）在猫中诱导的疾病与HIV相似，其特征在于伴随CD 4（+）细胞进行性下降的长期无症状阶段，最终导致AIDS。在本研究中，我们证明，FIV是更容易从CD8（+）T细胞耗尽的外周血单个核细胞（PBMC）的FIV感染的猫比从unfractionated PBMC培养分离。此外，从PBMC培养物中显示抗FIV活性的FIV阳性猫中分离的CD8（+）细胞在体外抑制FCD 4E细胞的FIV感染。在FIV阴性猫中未发现抗FIV活性，也不是FIV急性感染猫的特征，但在大多数慢性感染、临床无症状和有症状的猫中存在。在急性期FIV感染期间，血浆和细胞相关病毒血症的减少似乎先于循环中CD8（+）抗FIV细胞的出现。总之，本研究证明了慢性FIV感染猫中的CD8（+）T细胞群能够抑制培养的PBMC中的FIV复制。抗FIV CD8（+）细胞在感染和疾病进展的免疫发病机制中的重要性尚未确定。
Human immunodeficiency virus (HIV) causes a long, asymptomatic infection characterized by normal to elevated numbers of circulating CD8(+) cells and a progressive decline in CD4(+) cells. It has been speculated that HIV-specific antiviral activity driven by CD8(+) T cells may control viral replication during this period and maintain the clinically asymptomatic stage of disease. The disease induced in cats by feline immunodeficiency virus (FIV) is similar to HIV in that it is characterized by a long asymptomatic stage with a progressive decline in CD4(+) cells, culminating in AIDS. In the present study, we demonstrate that FIV is more readily isolated from CD8(+) T-cell-depleted peripheral blood mononuclear cells (PBMC) of FIV-infected cats than from unfractionated PBMC cultures. In addition, CD8(+) cells isolated from FIV-positive cats demonstrating anti-FIV activity in PBMC cultures inhibit FIV infection of FCD4E cells in vitro. Anti-FIV activity is not found in FIV-negative cats and is not characteristic of cats acutely infected with FIV but is present in the majority of chronically infected, clinically asymptomatic and symptomatic cats. Decreases in plasma and cell-associated viremia during the acute-stage FIV infection appears to precede the appearance of CD8(+) anti-FIV cells in the circulation. In summary, this study demonstrates a population(s) of CD8(+) T cells in chronically FIV-infected cats capable of suppressing FIV replication in cultured PBMC. The significance of anti-FIV CD8(+) cells in the immunopathogenesis of the infection and disease progression has yet to be determined.