Protein transport in nanoporous membranes modified with self-assembled monolayers of functionalized thiols
Protein transport in nanoporous membranes modified with self-assembled monolayers of functionalized thiols
复制标题
DOI：
10.1021/la011250b
复制
发表时间：
2002-06-11
期刊：
影响因子：
3.9
通讯作者：
Stroeve, P
中科院分区：
文献类型：
--
作者：
Chun, KY;Stroeve, P
利用表面修饰的纳米孔聚碳酸酯径迹蚀刻(PCTE)膜来控制外部pH和离子强度来分离蛋白质。在化学镀金表面用自组装硫醇(HSC10H20COOH)单分子膜修饰PCTE多孔膜。表面改性膜的水力孔径为8.7 nm。用两种相对分子质量相近的蛋白质--牛血清白蛋白(BSA)和牛血红蛋白(BHB)作为渗透剂。BSA和BHB通过膜的通量在蛋白质的等电点(PI)处出现最大值。当pH值高于和低于等电点时，蛋白质、它们的反离子和孔表面之间的电荷相互作用导致通量下降。在膜上施加不同的离子强度会导致渗透流动，导致蛋白质通量的显着增加，并增强BSA对BHB的选择性。在蛋白质分离实验中，BSA和BHB通量比观察到的通量大近3倍，没有离子强度差异。
Control of external pH and ionic strength is used to separate proteins with surface-modified, nanoporous polycarbonate track etched (PCTE) membranes. The porous PCTE membranes were modified with monolayers of self-assembled thiols (HSC10H20COOH) on electroless gold. The hydraulic radius of the pores in the surface-modified membranes was 8.7 nm. Two proteins of nearly identical molecular weight, bovine serum albumin (BSA) and bovine hemoglobin (BHb), were used as the permeants. The fluxes of BSA and BHb through the membranes show maximum values at the isoelectric points (pI) of the proteins. At pH values above and below the pI, charge interactions between the proteins, their counterions, and the pore surface leads to a decrease in flux. The imposition of a difference in ionic strength across the membrane causes osmotic flow and leads to a significant increase in the protein fluxes and an enhancement of the selectivity of BSA over BHb. In protein separation experiments, the BSA and BHb fluxes are nearly 3 times larger than those observed with no ionic strength difference.