Upregulation of miR-375 inhibits human liver cancer cell growth by modulating cell proliferation and apoptosis via targeting ErbB2
Upregulation of miR-375 inhibits human liver cancer cell growth by modulating cell proliferation and apoptosis via targeting ErbB2
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DOI：
10.3892/ol.2018.9011
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发表时间：
2018-09-01
期刊：
影响因子：
2.9
通讯作者：
Ding, Yan
中科院分区：
文献类型：
--
作者：
Li, Lina;Jia, Liping;Ding, Yan
microRNA（miRNA/miRs）是一类小的非编码RNA，通过调节癌基因的表达在肿瘤发生中发挥重要的生物学作用，可用于人类癌症的诊断和治疗。miR-375在许多类型的肿瘤中表现出异常表达水平;然而，miR-375在人肝细胞癌（HCC）中的生物学作用仍不完全。本研究调查了miR-375在人HCC组织和人肝癌细胞系中的表达;逆转录定量聚合酶链反应分析的结果表明，与正常组织和PHH细胞相比，miR-375在组织和肝癌细胞系中的表达显著降低。另外的研究表明，miR-375的上调通过调节细胞凋亡来抑制人肝癌细胞生长。研究还发现受体酪氨酸蛋白激酶erbB-2（ErbB 2）基因是miR-375的直接靶基因，ErbB 2的调控与人肝癌的生长有关。因此，本研究表明miR-375在人肝癌组织和细胞系中的表达水平均低于正常组织和PHH细胞，miR-375的诱导表达可能通过靶向ErbB 2基因调控人肝癌细胞的功能，这可能会在未来改善肝癌患者的诊断和治疗。
microRNAs (miRNA/miRs) are a class of small non-coding RNAs; they serve important biological roles in tumorigenesis through the regulation of oncogene expression, and they may be used for the diagnosis and treatment of human cancer. miR-375 was identified to exhibit abnormal expression levels in a number of types of tumor; however, the biological role of miR-375 in human hepatocellular carcinoma (HCC) remains incompletely characterized. The present study investigated the expression of miR-375 in human HCC tissues and human liver cancer cell lines; results from a reverse transcription quantitative polymerase chain reaction analysis indicated that the expression of miR-375 was significantly decreased in tissues and live cancer cell lines, compared with normal tissues and PHH cells. Additional studies demonstrated that the upregulation of miR-375 inhibited human liver cancer cell growth via regulation of cell apoptosis. It was also revealed that the receptor tyrosine-protein kinase erbB-2 (ErbB2) gene was a direct target gene of miR-375, and that the regulation of ErbB2 was associated with the human liver cancer growth. Therefore, the present study demonstrated that miR-375 was expressed at low levels both in human HCC tissues and cell line, compared with normal tissues and PHH cells, and that the induction of miR-375 expression may regulate human liver cancer cell function through targeting the ErbB2 gene, which may potentially improve the diagnosis and treatment of patients with HCC in the future.