A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway.
A plant growth-promoting bacteria Priestia megaterium JR48 induces plant resistance to the crucifer black rot via a salicylic acid-dependent signaling pathway.
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DOI：
10.3389/fpls.2022.1046181
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发表时间：
2022
影响因子：
5.6
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中科院分区：
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油菜黄单胞菌白菜黑腐病是十字花科植物的主要病害之一。利用有益的微生物来控制这种疾病是有希望的。在前期工作中，我们从菜地中分离到一株细菌（JR 48）。在此，我们证实了JR 48在植物中的植物生长促进（PGP）作用，并将JR 48鉴定为巨大锯木霉菌株。我们发现JR48能够诱导植物对Xcc的抗性，并引发植物防御反应，包括过氧化氢（H2O2）积累和胼胝质沉积，以及防御相关基因的表达增加。此外，JR48促进木质素的生物合成，增加游离水杨酸（SA）的积累以及病程相关（PR）基因的表达。最后，我们证实JR48诱导的植物抗性和防御反应需要SA信号通路。总之，我们的研究结果表明，JR 48促进植物生长，诱导植物对十字花科黑腐病的抗性可能是通过加强SA积累和响应，突出了其作为一种新型的生物防治剂的潜力，在未来。
Xanthomonas campestris pv. campestris (Xcc)-induced black rot is one of the most serious diseases in cruciferous plants. Using beneficial microbes to control this disease is promising. In our preliminary work, we isolated a bacterial strain (JR48) from a vegetable field. Here, we confirmed the plant-growth-promoting (PGP) effects of JR48 in planta, and identified JR48 as a Priestia megaterium strain. We found that JR48 was able to induce plant resistance to Xcc and prime plant defense responses including hydrogen peroxide (H2O2) accumulation and callose deposition with elevated expression of defense-related genes. Further, JR48 promoted lignin biosynthesis and raised accumulation of frees salicylic acid (SA) as well as expression of pathogenesis-related (PR) genes. Finally, we confirmed that JR48-induced plant resistance and defense responses requires SA signaling pathway. Together, our results revealed that JR48 promotes plant growth and induces plant resistance to the crucifer black rot probably through reinforcing SA accumulation and response, highlighting its potential as a novel biocontrol agent in the future.