Detection of molecular binding via charge-induced mechanical response of optical fibers.
Detection of molecular binding via charge-induced mechanical response of optical fibers.
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DOI：
10.1039/c4sc01188k
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发表时间：
2014
期刊：
影响因子：
8.4
通讯作者：
Tao N
中科院分区：
文献类型：
--
作者：
Guan Y;Shan X;Wang S;Zhang P;Tao N
我们报道了一种电荷敏感的光学检测技术，用于分子相互作用的无标记研究。传统的无标记光学检测技术在很大程度上依赖于对分子质量的检测，而分子质量对小分子不敏感。相比之下，目前的技术检测分子的电荷，其中的信号不会随着分子的大小而减弱，因此能够研究小分子。此外，该技术与标准微板平台兼容，适合高通量筛选候选药物。利用该技术，我们检测了0.2nM的抗牛血清白蛋白抗体和15μM的抗癌药物(伊马替尼)。实现的有效电荷检测下限为~0.25电子电荷/μ~2，相当于~0.3fg/mm~2，比传统的无标记光学检测方法高出一个数量级。
We report a charge sensitive optical detection technique for label-free study of molecular interactions. Traditional label-free optical detection techniques largely rely on the detection of the mass of a molecule, which are insensitive to small molecules. In contrast, the present technique detects the charge of a molecule, where the signal does not diminish with the size of the molecule, thus capable for studying small molecules. In addition, the technique is compatible with the standard microplate platform, making it suitable for high-throughput screening of drug candidates. Using the technique, we have detected 0.2 nM anti-BSA and 15 μM anti-cancer drug (imatinib) with an enzyme modified surface. The achieved effective charge detection limit is ~0.25 electron charge/μm2, corresponding to ~0.3 fg/mm2 for imatinib, which is orders of magnitude better than traditional label-free optical detection methods.