Structural elucidation of the extracellular and cell-wall teichoic acids of Staphylococcus epidermidis RP62A, a reference biofilm-positive strain
Structural elucidation of the extracellular and cell-wall teichoic acids of Staphylococcus epidermidis RP62A, a reference biofilm-positive strain
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DOI：
10.1016/j.carres.2004.03.017
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发表时间：
2004-06-01
影响因子：
3.1
通讯作者：
Jabbouri, S
中科院分区：
文献类型：
--
作者：
Sadovskaya, I;Vinogradov, E;Jabbouri, S
粘附于人工表面并形成生物膜的能力被认为是表皮葡萄球菌的毒力因子，表皮葡萄球菌是引起伤害性感染的主要原因之一，尤其是与植入医疗器械相关的伤害性感染。细胞壁磷壁酸在葡萄球菌生物膜形成中起重要作用。本文报道了S.通过NMR光谱和毛细管电泳-质谱法研究了一种参考生物膜阳性菌株epidermidis RP 62 A。它们的结构被发现是（1 -> 3）-连接的聚（甘油磷酸酯），在甘油残基的2-位被α-Glc、α-GlcNAc、D-Ala和α-Glc 6AIa取代。糖羟基的D-丙氨酰酰化似乎是来自葡萄球菌的磷壁酸的新结构特征。(C)2004爱思唯尔有限公司保留所有权利。
The ability to adhere to artificial surfaces and form biofilms is considered as a virulence factor of Staphylococcus epidermidis, one of the major causes of nocosomial infections, especially those related to implanted medical devices. Cell-wall teichoic acid is known to play an important role in biofilm formation of staphylococci. The structure of the cell wall and extracellular teichoic acids of S. epidermidis RP62A, a reference biofilm-positive strain, was studied by NMR spectroscopy and capillary electrophoresis-mass spectrometry. Their structures were found to be a (1 --> 3)-linked poly(glycerol phosphate), substituted at the 2-position of glycerol residues with alpha-Glc, alpha-GlcNAc, D-Ala and alpha-Glc6AIa. D-Alanyl acylation of a sugar hydroxyl group seems to be a novel structural feature of teichoic acids from staphylococci. (C) 2004 Elsevier Ltd. All rights reserved.