Combinatorial screening of osteoblast response to 3D calcium phosphate/poly(∈-caprolactone) scaffolds using gradients and arrays
Combinatorial screening of osteoblast response to 3D calcium phosphate/poly(∈-caprolactone) scaffolds using gradients and arrays
复制标题
DOI：
10.1016/j.biomaterials.2010.10.043
复制
发表时间：
2011-02-01
期刊：
影响因子：
14
通讯作者：
Simon, Carl G., Jr.
中科院分区：
文献类型：
--
作者：
Chatterjee, Kaushik;Sun, Limin;Simon, Carl G., Jr.
需要组合和高通量的方法来筛选细胞-生物材料相互作用，以最大限度地在支架中产生组织。目前的方法采用平面二维（2D）格式，尽管三维（3D）支架更能代表体内的组织环境，并且细胞对二维基质和三维支架的地形差异有反应。因此，开发了三维多孔支架组合文库，并用于筛选纳米无定形磷酸钙（nACP）颗粒对成骨细胞反应的影响。增加聚己内酯（PCL）支架中nACP的含量可促进成骨细胞的粘附和增殖。含有nacp的支架释放钙和磷酸盐离子，这是已知的激活成骨细胞功能。支架文库以梯度和阵列两种格式制备，阵列的nACP对成骨细胞增殖的影响程度大于梯度。阵列反应的增强可以用细胞培养设计、扩散效应和“支架质量与培养基”比例的差异来解释。这些结果引入了一种梯度文库方法来筛选大孔3D支架，并证明了nACP颗粒的包裹性增强了3D支架中成骨细胞的增殖。此外，梯度和阵列的比较表明，梯度在检测支架组成对细胞粘附的影响方面更敏感（短时间点，1天），而阵列在检测细胞增殖的影响方面更敏感（长时间点，14天）。Elsevier Ltd.出版。
There is a need for combinatorial and high-throughput methods for screening cell-biomaterial interactions to maximize tissue generation in scaffolds. Current methods employ a flat two-dimensional (2D) format even though three-dimensional (3D) scaffolds are more representative of the tissue environment in vivo and cells are responsive to topographical differences of 2D substrates and 3D scaffolds. Thus, combinatorial libraries of 3D porous scaffolds were developed and used to screen the effect of nano-amorphous calcium phosphate (nACP) particles on osteoblast response. Increasing nACP content in poly (is an element of-caprolactone) (PCL) scaffolds promoted osteoblast adhesion and proliferation. The nACP-containing scaffolds released calcium and phosphate ions which are known to activate osteoblast function. Scaffold libraries were fabricated in two formats, gradients and arrays, and the magnitude of the effect of nACP on osteoblast proliferation was greater for arrays than gradients. The enhanced response in arrays can be explained by differences in cell culture designs, diffusional effects and differences in the ratio of "scaffold mass to culture medium". These results introduce a gradient library approach for screening large pore 3D scaffolds and demonstrate that inclusion of the nACP particles enhances osteoblast proliferation in 3D scaffolds. Further, comparison of gradients and arrays suggests that gradients were more sensitive for detecting effects of scaffold composition on cell adhesion (short time points, 1 day) whereas arrays were more sensitive at detecting effects on cell proliferation (longer time points, 14 day). Published by Elsevier Ltd.