A Study of the Effects of Plasma Surface Treatment on Lipid Bilayers Self-Spreading on a Polydimethylsiloxane Substrate under Different Treatment Times
A Study of the Effects of Plasma Surface Treatment on Lipid Bilayers Self-Spreading on a Polydimethylsiloxane Substrate under Different Treatment Times
复制标题
不同处理时间下等离子体表面处理对聚二甲基硅氧烷基底上脂质双层自铺展的影响研究
DOI：
10.1021/acs.langmuir.1c01319
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发表时间：
2021
期刊：
影响因子：
3.9
通讯作者：
Zugui Peng,Kenta Shimba,Yoshitaka Miyamoto,Tohru Yagi
中科院分区：
文献类型：
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作者：
Sugiyama Hironobu;Miyazaki Masayoshi;Sasase Masato;Kitano Masaaki;Hosono Hideo;Zugui Peng,Kenta Shimba,Yoshitaka Miyamoto,Tohru Yagi
等离子体处理的聚二甲基硅氧烷（PDMS）支持的脂质双层被用作研究细胞膜特性的功能工具和生物技术应用的平台。自铺展是形成脂质双层的通用方法。然而，很少有研究集中在等离子体处理对自扩散脂双层形成的影响。在本文中，我们进行了脂双层自铺展在PDMS表面与不同的处理时间。利用原子力显微镜和扫描电镜对不同处理时间的PDMS表面进行表征，并利用共聚焦显微镜研究了等离子体处理对PDMS表面脂双层自铺展行为的影响。随着等离子体处理时间的延长，脂双层的前沿速度增加。通过扩展的DLVO模型的理论分析，我们发现最可能的原因是PDMS表面和脂质双层之间的水化排斥能的速度变化。此外，膜叶的生长行为的影响下的自蔓延脂双层的PDMS表面的形貌变化，导致等离子体处理。我们的研究结果表明，可以通过改变等离子体处理时间来控制自扩散脂质双层的生长。
Plasma-treated poly(dimethylsiloxane) (PDMS)-supported lipid bilayers are used as functional tools for studying cell membrane properties and as platforms for biotechnology applications. Self-spreading is a versatile method for forming lipid bilayers. However, few studies have focused on the effect of plasma treatment on self-spreading lipid bilayer formation. In this paper, we performed lipid bilayer self-spreading on a PDMS surface with different treatment times. Surface characterization of PDMS treated with different treatment times is evaluated by AFM and SEM, and the effects of plasma treatment of the PDMS surface on lipid bilayer self-spreading behavior is investigated by confocal microscopy. The front-edge velocity of lipid bilayers increases with the plasma treatment time. By theoretical analyses with the extended-DLVO modeling, we find that the most likely cause of the velocity change is the hydration repulsion energy between the PDMS surface and lipid bilayers. Moreover, the growth behavior of membrane lobes on the underlying self-spreading lipid bilayer was affected by topography changes in the PDMS surface resulting from plasma treatment. Our findings suggest that the growth of self-spreading lipid bilayers can be controlled by changing the plasma treatment time.