Survival of blastocysts in the uteri of ovariectomized mice.
Survival of blastocysts in the uteri of ovariectomized mice.
复制标题
卵巢切除小鼠子宫中囊胚的存活率。
DOI：
10.1530/jrf.0.0170515
复制
发表时间：
1968
期刊：
影响因子：
--
通讯作者：
G. Greenwald
中科院分区：
文献类型：
--
作者：
H. Weitlauf;G. Greenwald
总结。成年小鼠于术后第4天(第1天=阴道插管)摘除卵巢，不作进一步处理。未植入(延迟)的囊胚是在交配后5至40天从这些雌性动物身上获得的。通过确定移植给假孕第四天的受者后发育成正常胎儿的比例来测试他们的生存能力。结果发现，移植囊胚在延迟0~10天后发育成胎儿的比例相似。经过10天以上的延迟后，能够发育为正常胚胎的囊胚比例下降；移植后5~15天发育30~32%，移植20天18%，移植30天3%。这一发现表明，在没有卵巢激素的情况下，胚泡可以被推迟，但仍然能够发育成正常的胎儿，这表明黄体酮对小鼠的胚泡存活并不是必不可少的。
Summary. Mature mice were ovariectomized on the 4th day post coitum (Day 1 = vaginal plug) and given no further treatment. Unimplanted (delayed) blastocysts were recovered from these females between 5 and 40 days post coitum. They were tested for viability by determining the proportion that developed into normal foetuses following transfer to Day-4 pseudopregnant recipients. It was found that the proportion of transferred blastocysts developing into foetuses was similar after delays of from 0 to 10 days. After delays of more than 10 days, the proportion of blastocysts that were capable of developing into normal foetuses decreased; 30 to 32% developed following transfer on Days 5 to 15, 18% with transfer on Day 20, and 3% with transfer on Day 30. The finding that blastocysts can be delayed in the absence of ovarian hormones and yet remain capable of developing into normal foetuses demonstrates that progesterone is not essential for blastocyst survival in mice.