Analysis of plasma microRNA expression profiles in male textile workers with noise-induced hearing loss
Analysis of plasma microRNA expression profiles in male textile workers with noise-induced hearing loss
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DOI：
10.1016/j.heares.2015.08.003
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发表时间：
2016-03-01
期刊：
影响因子：
2.8
通讯作者：
Zhu, Bao-Li
中科院分区：
文献类型：
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作者：
Ding, Lu;Liu, Jing;Zhu, Bao-Li
背景：循环 microRNA（miRNA）作为生理和病理条件的非侵入性生物标志物引起了人们的兴趣，其可应用于噪声性听力损失（NIHL）。然而，目前还没有流行病学研究检验 NIHL 或噪声暴露对 miRNA 表达谱的潜在影响。 目标：我们试图鉴定永久性 NIHL 相关 miRNA，并预测编码所示 miRNA 的推定基因的生物学功能。 方法：在发现阶段，我们使用微阵列测定法检测 10 名听力正常的噪声暴露个体和 10 名 NIHL 患者的混合血浆样本之间的 miRNA 表达谱。此外，我们对这 20 名工人中的 6 个候选 miRNA 进行了初步验证。随后，选择了 3 个 miRNA 在 23 名听力正常的非暴露个体和 46 名暴露于噪声的纺织工人中进行扩展验证，其中包括 23 名听力正常的暴露于噪声的工人和 23 名 NIHL 患者。此外，我们使用基因本体论（GO）功能富集分析预测了假定目标基因的生物学功能。结果：在发现阶段，与正常听力的噪声暴露相比，73个miRNA在NIHL患者中表现出至少1.5倍的差异表达。初步验证中，与噪声暴露相比，NIHL患者血浆中miR-16-5p、miR-24-3p、miR-185-5p和miR-451a水平均上调（P < 0.001）。在扩展验证阶段，与未暴露组相比，暴露组血浆中miR-24、miR-185-5p和miR-451a水平均显着下调（P < 0.001）。与噪声暴露相比，NIHL患者血浆中miR-185-5p和miR-451a水平略有升高（P < 0.001），这与初步验证和微阵列分析的结果一致。结论：这两种血浆miRNA可能是指示噪声暴露反应的生物标志物。然而，还需要进一步的研究来证明 miRNA 变化与噪声暴露之间的因果关系，并确定这两种 miRNA 是否是噪声暴露的明确生物标志物。 (C) 2015 Elsevier B.V. 保留所有权利。
Background: Circulating microRNAs (miRNAs) have attracted interests as non-invasive biomarkers of physiological and pathological conditions, which may be applied in noise-induced hearing loss (NIHL). However, no epidemiology studies have yet examined the potential effects of NIHL or noise exposure on miRNA expression profiles.Objectives: We sought to identify permanent NIHL-related miRNAs and to predict the biological functions of the putative genes encoding the indicated miRNAs.Methods: In the discovery stage, we used a microarray assay to detect the miRNA expression profiles between pooled plasma samples from 10 noise-exposed individuals with normal hearing and 10 NIHL patients. In addition, we conducted a preliminary validation of six candidate miRNAs in the same 20 workers. Subsequently, three miRNAs were selected for expanded validation in 23 non-exposed individuals with normal hearing and 46 noise-exposed textile workers which including 23 noise-exposed workers with normal hearing and 23 NIHL patients. Moreover, we predicted the biological functions of the putative target genes using a Gene Ontology (GO) function enrichment analysis.Results: In the discovery stage, compared with the noise exposures with normal hearing, 73 miRNAs demonstrated at least a 1.5-fold differential expression in the NIHL patients. In the preliminary validation, compared with the noise exposures, the plasma levels of miR-16-5p, miR-24-3p, miR-185-5p and miR-451a were all upregulated (P < 0.001) in the NIHL patients. In the expanded validation stage, compared with the non-exposures, the plasma levels of miR-24, miR-185-5p and miR-451a were all significantly downregulated (P < 0.001) in the exposures. And compared with the noise exposures, the plasma levels of miR-185-5p and miR-451a were slightly elevated (P < 0.001) in the NIHL patients, which were consistent with the results of preliminary validation and microarray analysis.Conclusion: The two indicated plasma miRNAs may be biomarkers of indicating responses to noise exposure. However, further studies are necessary to prove the causal association between miRNAs changes and noise exposure, and to determine whether these two miRNAs are clear biomarkers to noise exposure. (C) 2015 Elsevier B.V. All rights reserved.