Identification of ecdysteroidogenic enzyme genes and their expression during pupal diapause in the cabbage armyworm, Mamestra brassicae.
Identification of ecdysteroidogenic enzyme genes and their expression during pupal diapause in the cabbage armyworm, Mamestra brassicae.
复制标题
甘蓝粘虫蜕皮类固醇生成酶基因的鉴定及其在蛹滞育期间的表达。
DOI：
10.1111/imb.12291
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发表时间：
2017
影响因子：
2.6
通讯作者：
Kataoka H
中科院分区：
文献类型：
--
作者：
Ogihara MH;Ikeda H;Yamada N;Hikiba J;Nakaoka T;Fujimoto Y;Suzuki Y;Saito K;Mizoguchi A;Kataoka H
在这项研究中，我们在甘蓝粘虫（Mamestra brassicae）中鉴定了体外甾体生成酶，并证明这些基因在滞育期间表达减少。有些昆虫为了在恶劣的环境中生存，会暂时停止发育，即滞育。滞育蛹的蜕皮激素滴度降低。brassicae;因此，卵巢甾体形成可能受到滞育特异性机制的抑制。目的：阐明异卵甾体酶基因在滞育过程中的表达变化。首先，我们鉴定了7种蜕皮甾体生成酶的基因：Neverland、Non - molting glossy （Nm‐g）、CYP307A1 （Spook）、CYP306A1 （Phantom）、CYP302A1 （Disembodied）、CYP315A1 （Shadow）和CYP314A1 （Shade）。在果蝇施耐德2 （S2）细胞中异种表达的酶促实验和mRNA分布分析表明，所鉴定的基因为m的体外甾体生成酶。brassicae。比较了不同蛹期前胸腺在滞育过程中这些异卵甾体生成酶基因的表达水平。蛹化后，滞育终蛹与未滞育终蛹的体外甾体生成酶基因表达水平相近。这些基因在滞育启动后均表现出表达减少的趋势。在滞育后静息期，表达量立即增加。这些结果表明，体外甾体生成酶基因表达的减少抑制体外甾体形成，并维持滞育期间的发育停滞。
In this study, we identified ecdysteroidogenic enzymes in the cabbage armyworm,Mamestra brassicae, and demonstrated reduced expression of these genes during diapause. Some insects employ a temporary developmental arrest, diapause, to survive in severe environments. The titres of the moulting hormone ecdysteroid were reduced in diapause pupae ofM. brassicae; therefore, ecdysteroidogenesis might be suppressed by a diapause‐specific mechanism. To clarify expression changes of ecdysteroidogenic enzyme genes during diapause inM. brassicae, we first identified the genes for seven ecdysteroidogenic enzymes: Neverland, Non‐molting glossy (Nm‐g), CYP307A1 (Spook), CYP306A1 (Phantom), CYP302A1 (Disembodied), CYP315A1 (Shadow) and CYP314A1 (Shade). Enzymatic assays using heterologous expression inDrosophilaSchneider 2 (S2) cells and analysis of mRNA distribution indicated that the identified genes were ecdysteroidogenic enzymes ofM. brassicae. Expression levels of these ecdysteroidogenic enzyme genes were compared between prothoracic glands in different pupal stages throughout diapause. Immediately after pupation, diapause‐destined pupae showed similar expression levels of ecdysteroidogenic enzyme genes to those of nondiapause pupae. All of these genes showed reduced gene expression after diapause initiation. Expression was immediately increased in diapause‐destined pupae at the postdiapause quiescence phase. These results indicate that reduced expression of ecdysteroidogenic enzyme genes suppresses ecdysteroidogenesis and maintains developmental arrest during diapause.