PLASMODIUM-FALCIPARUM - DETECTION OF ANTIFOLATE RESISTANCE BY MUTATION-SPECIFIC RESTRICTION ENZYME DIGESTION
PLASMODIUM-FALCIPARUM - DETECTION OF ANTIFOLATE RESISTANCE BY MUTATION-SPECIFIC RESTRICTION ENZYME DIGESTION
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DOI：
10.1006/expr.1995.1060
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发表时间：
1995-05-01
影响因子：
2.1
通讯作者：
MAZABRAUD, A
中科院分区：
文献类型：
--
作者：
DEPECOULAS, PE;BASCO, LK;MAZABRAUD, A
我们在这里描述了一个快速的程序来预测恶性疟原虫对乙胺嘧啶或环胍的耐药性。该方法包括通过PCR扩增DHFR基因，然后通过限制性内切酶消化密码子16和108。使用三种不同的酶来切割野生型、108-苏氨酸突变体和108-天冬酰胺突变体基因。由于迄今为止发现的每种天然抗叶酸剂耐药分离株都在密码子108处携带突变，因此确定该密码子的性质可以预测任何恶性疟原虫分离株的敏感性。(C)出版社：Academic Press
We describe here a rapid procedure to predict the resistance of Plasmodium falciparum to pyrimethamine or cycloguanil. The method consists of amplification by PCR of the DHFR gene followed by restriction enzyme digestion of codons 16 and 108. Three different enzymes are used to cut the wild-type, 108-threonine mutant, and 108-asparagine mutant gene. Since every natural antifolate-resistant isolate identified until now carries a mutation in codon 108, determination of the nature of this codon can predict the sensitivity of any P. falciparum isolate. (C) 1995 Academic Press, Inc.