Tracing cellular dynamics in tissue development, maintenance and disease
Tracing cellular dynamics in tissue development, maintenance and disease
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DOI：
10.1016/j.ceb.2016.07.001
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发表时间：
2016-12-01
影响因子：
7.5
通讯作者：
Simons, Benjamin D.
中科院分区：
文献类型：
--
作者：
Rulands, Steffen;Simons, Benjamin D.
细胞增殖和分化的协调对于组织的发育和维持至关重要，而其失调则是向疾病状态转变的基础。通过将谱系追踪与转录分析和基于标记的分析相结合，统计方法可以深入了解干细胞及其发育前体的动态。这些研究为分子异质性和命运启动提供了证据，并揭示了随机性在干细胞命运中的作用，重新聚焦于调控机制的研究。同时，他们提供了一个定量平台来研究疾病的发生和进展。在这里，我们回顾了定量谱系追踪策略如何塑造我们对组织发育、维持和疾病的细胞机制的理解。
The coordination of cell proliferation and differentiation is central to the development and maintenance of tissues, while its dysregulation underlies the transition to diseased states. By combining lineage tracing with transcriptional profiling and marker-based assays, statistical methods are delivering insights into the dynamics of stem cells and their developmental precursors. These studies have provided evidence for molecular heterogeneity and fate priming, and have revealed a role for stochasticity in stem cell fate, refocusing the search for regulatory mechanisms. At the same time, they present a quantitative platform to study the initiation and progression of disease. Here, we review how quantitative lineage tracing strategies are shaping our understanding of the cellular mechanisms of tissue development, maintenance and disease.