Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR
Impact of competitive inhibition and sequence variation upon the sensitivity of malaria PCR
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DOI：
10.1128/jcm.02145-06
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发表时间：
2007-05-01
影响因子：
9.4
通讯作者：
Sloots, Theo P.
中科院分区：
文献类型：
--
作者：
Bialasiewicz, Seweryn;Whiley, David M.;Sloots, Theo P.
利用通用引物和种特异性引物对疟原虫进行实时PCR检测，比较影响检测灵敏度降低的变量。低聚物靶点的序列变化和通用引物混合物中双种模板的竞争性抑制被发现降低了检测灵敏度。
Real-time PCR assays for Plasmodium species utilizing universal and species-specific primers were compared to investigate variables influencing decreased assay sensitivity. Sequence variation in oligomer targets and competitive inhibition of dual-species templates in universal-primer mixes were found to decrease assay sensitivity.