Endocytic Active Zones: Hot Spots for Endocytosis in Vertebrate Neuromuscular Terminals
Endocytic Active Zones: Hot Spots for Endocytosis in Vertebrate Neuromuscular Terminals
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DOI：
10.1523/jneurosci.19-12-04855.1999
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发表时间：
1999-06
期刊：
影响因子：
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通讯作者：
H. Teng;J. C. Cole;R. Roberts;R. S. Wilkinson
中科院分区：
文献类型：
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作者：
H. Teng;J. C. Cole;R. Roberts;R. S. Wilkinson
我们使用了一种灵敏的活性依赖的探针，磺胺类霍丹明101(SR101)，来观察蛇运动神经末梢内的内吞事件。在降温条件下进行非常短暂的神经刺激后，SR101仅在位于每个末端突触前膜内的点状位置可见。它们的数目(∼26个位点/单位)和它们的位置(与突触后折叠相一致)与蛇运动终末活动区的数目和位置相似，提示在这些刺激条件下胞吐和内吞作用之间存在空间联系。随着刺激时间的延长，更大的含SR101的结构出现在边缘。因此，内吞作用最初发生在不同的部位，我们称之为“内吞活跃区”，而进一步的刺激则招募第二种内吞范例。
We have used a sensitive activity-dependent probe, sulforhodamine 101 (SR101), to view endocytic events within snake motor nerve terminals. After very brief neural stimulation at reduced temperature, SR101 is visualized exclusively at punctate sites located just inside the presynaptic membrane of each terminal bouton. The number of sites (∼26 sites/bouton) and their location (in register with postsynaptic folds) are similar to the number and location of active zones in snake motor terminals, suggesting a spatial association between exocytosis and endocytosis under these stimulus conditions. With more prolonged stimulation, larger SR101-containing structures appear at the bouton margins. Thus endocytosis occurs initially at distinct sites, which we call “endocytic active zones,” whereas further stimulation recruits a second endocytic paradigm.