The activation of memory CD4+ T cells and CD8+ T cells in patients with multiple sclerosis
The activation of memory CD4+ T cells and CD8+ T cells in patients with multiple sclerosis
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DOI：
10.1016/j.jns.2005.02.013
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发表时间：
2005-08-15
影响因子：
4.4
通讯作者：
Posnett, DN
中科院分区：
文献类型：
--
作者：
Okuda, Y;Okuda, M;Posnett, DN
为了重新评估多发性硬化（MS）的临床过程和记忆T细胞的激活之间是否存在关联，我们采用五色流式细胞术研究了MS患者外周血和脑脊液（CSF）中T细胞的表型。对39例复发缓解型MS患者进行的横断面研究表明，与非活动期MS患者相比，活动期MS患者外周血和CSF中的CD 25（+）CD 45 RO（+）CD 4（+）CD 3（+）细胞百分比显著增加。对11例复发缓解型MS患者的纵向研究也显示，与缓解期相比，恶化期外周血中CD 25（+）CD 45 RO（+）CD 4（+）CD 3（+）细胞的百分比更高。另一方面，与疾病活动性无关，MS患者外周血中CD25（+）CD45RO（+）CD8（+）CD3（+）细胞的百分比显著高于健康对照受试者。活动期MS患者CSF中CD25（+）CD45RO（+）CD8（+）CD3（+）细胞百分比低于非活动期MS患者。这些结果表明记忆性CD4（+）T细胞的激活与MS的恶化有关，记忆性CD8（+）T细胞的激活反映了MS患者的全身免疫失调。记忆抗原对T细胞的短暂和持续激活可能参与MS的病程。（C）2005 Elsevier B.V.保留所有权利。
To reevaluate whether an association exists between the clinical course of multiple sclerosis (MS) and the activation of memory T cells, we investigated the phenotype of T cells in peripheral blood and cerebrospinal fluid (CSF) of patients with MS using five-color flow cytometry. A cross-sectional study with 39 relapsing -remitting MS patients demonstrated that the percentage of CD25(+)CD45RO(+)CD4(+)CD3(+) cells was significantly increased in peripheral blood as well as in CSF of active MS patients compared with inactive MS patients. A longitudinal study with 11 relapsing -remitting MS patients also showed a higher percentage of CD25(+)CD45RO(+)CD4(+)CD3(+) cells in peripheral blood at the phase of exacerbation than during remission. On the other hand, regardless of the disease activity, the percentage of CD25(+)CD45RO(+)CD8(+)CD3(+) cells in peripheral blood was significantly higher in patients with MS than in healthy control subjects. A lower percentage of CD25(+)CD45RO(+)CD8(+)CD3(+) cells in CSF was observed in active MS patients compared with inactive MS patients. These results suggest that the activation of memory CD4(+) T cells is associated with the exacerbation of MS and activation of memory CD8(+) T cells reflects systemic immunological dysregulation in MS patients. Transient as well as continuous activation of T cells by recall antigens may be involved in the disease course of MS. (C) 2005 Elsevier B.V. All rights reserved.