Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
Paradoxical electro-olfactogram responses in TMEM16B knock-out mice.
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TMEM16B 敲除小鼠中矛盾的电嗅觉反应。
DOI：
10.1093/chemse/bjad003
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发表时间：
2023
期刊：
影响因子：
3.5
通讯作者：
Menini,Anna
中科院分区：
文献类型：
--
作者：
Guarneri,Giorgia;Pifferi,Simone;Dibattista,Michele;Reisert,Johannes;Menini,Anna
Ca 2+激活的Cl '通道TMEM 16 B携带高达90%的嗅觉感觉神经元中由气味刺激诱发的转导电流，并控制动作电位放电的数量，从而控制动作电位序列的长度。功能丧失的方法显示，TMEM 16 B是嗅觉驱动的行为所必需的，例如跟踪不熟悉的气味。在这里，我们使用的电嗅觉图（EOG）技术，以调查的贡献TMEM 16 B的气味转导在整个嗅上皮。令人惊讶的是，我们发现Tmem 16 b基因敲除小鼠的EOG反应与野生型小鼠相比具有更大的幅度。此外，EOG反应的动力学在不存在TMEM 16 B的情况下更快，而适应重复刺激的能力在敲除小鼠中改变。Tmem 16 bknockout中较大的EOG响应可能是去除了Ca 2+激活的Cl <$电流的钳位和/或分流作用的结果，导致具有较小的转导电流但较大的发生器电位的矛盾。
The Ca2+-activated Cl¯ channel TMEM16B carries up to 90% of the transduction current evoked by odorant stimulation in olfactory sensory neurons and control the number of action potential firing and therefore the length of the train of action potentials. A loss of function approach revealed that TMEM16B is required for olfactory-driven behaviors such as tracking unfamiliar odors. Here, we used the electro-olfactogram (EOG) technique to investigate the contribution of TMEM16B to odorant transduction in the whole olfactory epithelium. Surprisingly, we found that EOG responses fromTmem16bknock out mice have a bigger amplitude compared to those of wild type. Moreover, the kinetics of EOG responses is faster in absence of TMEM16B, while the ability to adapt to repeated stimulation is altered in knock out mice. The larger EOG responses inTmem16bknock out may be the results of the removal of the clamping and/or shunting action of the Ca2+-activated Cl¯ currents leading to the paradox of having smaller transduction current but larger generator potential.