Hypochlorite modification of high density lipoprotein: effects on cholesterol efflux from J774 macrophages
Hypochlorite modification of high density lipoprotein: effects on cholesterol efflux from J774 macrophages
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DOI：
10.1016/s0014-5793(99)00677-8
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发表时间：
1999-06-11
期刊：
影响因子：
3.5
通讯作者：
Sattler, W
中科院分区：
文献类型：
--
作者：
Bergt, C;Reicher, H;Sattler, W
本研究旨在研究次氯酸盐（HOCl）修饰高密度脂蛋白亚类3 （HDL3）对永久性J774巨噬细胞去除细胞胆固醇能力的影响。我们的研究结果表明，HOCl（作为试剂添加或由髓过氧化物酶/H2O2/Cl-系统酶化产生）破坏HDL3的主要蛋白质成分载脂蛋白A-I。对天然HDL3和HOCl修饰HDL3的脂肪酸分析显示，两种主要脂质亚类（磷脂类和胆固醇类）中的不饱和脂肪酸都是HOCl攻击的目标。无论用试剂还是酶促生成的HOCl修饰脂蛋白，HOCl修饰导致J774细胞hdl3介导的胆固醇外排受损，重组HDL颗粒经过HOCl修饰后，胆固醇外排也减少，在低和生生性HOCl浓度下，巨噬细胞的胆固醇外排受损。(C) 1999年欧洲生化学会联合会。
The present study was aimed at investigating effects of hypochlorite (HOCl) modification of high density lipoproteins subclass 3 (HDL3) on their ability for cellular cholesterol removal from permanent J774 macrophages, Our findings indicate that HOCl (added as reagent or generated enzymatically by the myeloperoxidase/H2O2/Cl- system) damages apolipoprotein A-I, the major protein component of HDL3, Fatty acid analysis of native and HOCl-modified HDL3 revealed that unsaturated fatty acids in both major lipid subclasses (phospholipids and cholesteryl eaters) are targets for HOCl attack. HOCl modification resulted in impaired HDL3-mediated cholesterol efflux from J774 cells, regardless of whether reagent or enzymatically generated HOCl was used to modify the lipoprotein, Decreased cholesterol efflux was also observed after HOCl modification of reconstituted HDL particles, Impairment of cholesterol efflux from macrophages was noticed at low and physiologically occurring HOCl concentrations. (C) 1999 Federation of European Biochemical Societies.