Lighting the way to see inside the live cell with luminescent transition metal complexes
Lighting the way to see inside the live cell with luminescent transition metal complexes
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DOI：
10.1016/j.ccr.2012.03.018
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发表时间：
2012-08-01
影响因子：
20.6
通讯作者：
Williams, J. A. Gareth
中科院分区：
文献类型：
--
作者：
Baggaley, Elizabeth;Weinstein, Julia A.;Williams, J. A. Gareth
发光金属配合物在细胞成像中的应用是一个迅速兴起的领域。本文综述了该领域的背景，这种配合物可能提供的优势，超过传统的荧光成像探针，并成功的applications.We特别关注的各种应用所提供的正方形平面铂（II）配合物，包括它们在时间分辨发射成像显微镜（TREM）的使用所需的要求。这种新兴的技术利用了这种金属配合物的长发光寿命，大大扩展了传统的荧光寿命成像显微镜（FLIM）的范围，该显微镜通常在纳秒时间尺度上发射的荧光团上进行。还讨论了最近与d（6）金属离子，Ir（III），Ru（II）和Re（I）的配合物的例子。过渡金属络合物可以提供的众多应用-这不一定是可访问的有机标签-概述，并链接到物理性质以及标签和成像对象之间的相互作用的模式。(C)2012爱思唯尔有限公司版权所有。
The application of luminescent metal complexes to cell imaging is a rapidly emerging area. This review discusses the background to the field, the advantages that such complexes may offer over conventional fluorescent imaging probes, and the desirable requirements for successful applications.We particularly focus on a variety of applications that are offered by square planar platinum(II) complexes, including their use in time-resolved emission imaging microscopy (TREM). This emerging technique exploits the long luminescence lifetimes of such metal complexes to considerably extend the scope of conventional fluorescence lifetime imaging microscopy (FLIM), which is normally carried out on fluorophores that emit on the nanosecond timescale. Selected recent examples of complexes with d(6) metal ions, Ir(III), Ru(II) and Re(I), are also discussed. The multitude of applications that transition metal complexes can offer - and which are not necessarily accessible with organic labels - are outlined, and linked to photophysical properties as well as to the modes of interaction between the label and the imaged object. (C) 2012 Elsevier B.V. All rights reserved.