DIRECT EVIDENCE FOR THE ROLE OF LIGHT-MEDIATED GAS VESICLE COLLAPSE IN THE BUOYANCY REGULATION OF ANABAENA-FLOS-AQUAE (CYANOBACTERIA)
DIRECT EVIDENCE FOR THE ROLE OF LIGHT-MEDIATED GAS VESICLE COLLAPSE IN THE BUOYANCY REGULATION OF ANABAENA-FLOS-AQUAE (CYANOBACTERIA)
复制标题
DOI：
10.4319/lo.1984.29.4.0879
复制
发表时间：
1984-01-01
影响因子：
4.5
通讯作者：
WALSBY, AE
中科院分区：
文献类型：
--
作者：
OLIVER, RL;WALSBY, AE
本文定量测定了A. L.培养物中液泡体积和细胞团主要成分（蛋白质和碳水化合物）的变化。当它们从低光强度转移到高光强度时失去浮力的絮状水生物。假设密度为1300 kg。m-3的蛋白质和1600的碳水化合物，这些成分的变化所带来的压舱物的变化进行了计算，并与由液泡崩溃造成的压舱物的变化进行了比较。计算出的过剩密度的细丝也进行了比较，直接测量的细丝密度梯度的Percoll。密度的增加是由于气泡的减少。密度的微小变化可归因于蛋白质和碳水化合物的变化，但在没有气泡含量减少的情况下，这些变化不会引起浮力的损失。所述类型的分析可用于确定其他微生物浮力变化的原因，并可对从湖泊中收集的浮游植物进行分析。
Quantitative measurements were made of the changes in gas vacuole volume and the major components of cell mass (protein and carbohdyrate) on cultures of A. flos-aquae which lost buoyancy as they were shifted from low to high light intensity. Assuming densities of 1300 kg .cntdot. m-3 for protein and 1600 for carbohydrate, the change in ballast brought about by changes in these components were calculated and were compared with ballast changes resulting from gas vacuole collapse. The calculated excess density of filaments were also compared with direct measurements of filament density in gradients of Percoll. Increased density resulted from the loss of gas vacuoles. Small variations in density could be attributed to changes in protein and carbohydrate, but these would not have caused a loss of buoyancy in the absence of a decrease in gas vacuole content. Analyses of the type described can be used to determine the cause of buoyancy change in other microorganisms and can be performed on phytoplankton collected from lakes.