Evolutionary trade-offs at the Arabidopsis WRR4A resistance locus underpin alternate Albugo candida recognition specificities
Evolutionary trade-offs at the Arabidopsis WRR4A resistance locus underpin alternate Albugo candida recognition specificities
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DOI：
10.1101/2021.03.29.437434
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发表时间：
2021-03
期刊：
影响因子：
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通讯作者：
Baptiste Castel;Sebastian Fairhead;Oliver J. Furzer;A. Redkar;Shanshan Wang;V. Cevik;E. Holub;Jonathan D. G. Jones
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文献类型：
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作者：
Baptiste Castel;Sebastian Fairhead;Oliver J. Furzer;A. Redkar;Shanshan Wang;V. Cevik;E. Holub;Jonathan D. G. Jones
卵菌Albugo candida 会导致十字花科植物产生白锈病，包括蔬菜和油籽作物以及拟南芥等野生近缘种。来自拟南芥的新型白锈病抗性（WRR）基因为植物/寄生虫共同进化提供了新的见解。来自拟南芥登录 Col-0 的 WRR4A 对许多但不是所有白锈菌品种提供抗性，并编码核苷酸结合 (NB)、富含亮氨酸重复序列 (LRR) (NLR) 免疫受体蛋白。 Col-0 WRR4A 抗性被念珠菌小种 4 (AcEx1) 的 Col-0 强毒分离株破坏。我们在拟南芥种质 Oy-0 和其他对 AcEx1 具有完全抗性的种质中鉴定出了 WRR4A 的等位基因。 WRR4AOy-0 携带识别 AcEx1 所需的 C 端延伸，但会降低对 WRR4ACol-0 等位基因识别的多个效应子的识别。当 WRR4AOy-0 在油料作物亚麻荠中表达为转基因时，可赋予对 AcEx1 的完全抗性。意义 拟南芥抗性蛋白 WRR4A 等位基因的 C 端延伸改变了效应子识别特异性，使 WRR4AOy-0 等位基因能够赋予克服 WRR4ACol-0 等位基因的白假丝酵母菌种免疫力。这种抗性可以转移到产油作物亚麻荠身上。图文摘要
The oomycete Albugo candida causes white rust of Brassicaceae, including vegetable and oilseed crops, and wild relatives such as Arabidopsis thaliana. Novel White Rust Resistance (WRR)-genes from Arabidopsis enable new insights into plant/parasite co-evolution. WRR4A from Arabidopsis accession Col-0 provides resistance to many but not all white rust races, and encodes a nucleotide-binding (NB), leucine-rich repeat (LRR) (NLR) immune receptor protein. Col-0 WRR4A resistance is broken by a Col-0-virulent isolate of A. candida race 4 (AcEx1). We identified an allele of WRR4A in Arabidopsis accession Oy-0 and other accessions that confers full resistance to AcEx1. WRR4AOy-0 carries a C-terminal extension required for recognition of AcEx1, but reduces recognition of several effectors recognized by the WRR4ACol-0 allele. WRR4AOy-0 confers full resistance to AcEx1 when expressed as a transgene in the oilseed crop Camelina sativa. Significance A C-terminal extension in an allele of the Arabidopsis resistance-protein WRR4A changes effector recognition specificity, enabling the WRR4AOy-0 allele to confer immunity to Albugo candida races that overcome the WRR4ACol-0 allele. This resistance can be transferred to the oil-producing crop Camelina sativa. Graphical abstract