Effect of Activin A on In Vitro Development of Rat Preantral Follicles and Localization of Activin A and Activin Receptor II
Effect of Activin A on In Vitro Development of Rat Preantral Follicles and Localization of Activin A and Activin Receptor II
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DOI：
10.1095/biolreprod65.3.967
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发表时间：
2001-09
期刊：
影响因子：
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通讯作者：
Jia Zhao;M. Taverne;G. C. van der Weijden;M. Bevers;R. van den Hurk
中科院分区：
文献类型：
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作者：
Jia Zhao;M. Taverne;G. C. van der Weijden;M. Bevers;R. van den Hurk
从10日龄大鼠卵巢中机械分离出140 ~ 160 μm的前腔卵泡，在含有0、1、10、100 ng/ml激活素a的培养基中，4 ~ 6组培养6 d。激活素刺激（P < 0.05）前腔卵泡的生长，并促进了无卵母细胞的前腔卵泡细胞的增殖。此外，激活素处理诱导大多数卵泡形成腔样结构，并有助于在培养过程中维持这些卵泡的超微结构。激活素A还诱导了卵泡和卵母细胞成熟的进一步变化，如与卵母细胞接触的颗粒细胞的伸长和皮层颗粒向卵母细胞皮层的迁移。此外，利用逆转录聚合酶链反应，激活素和激活素受体II型（ActR II）的基因表达在卵母细胞和体细胞中都得到了证实，免疫组织化学研究表明，激活素和ActR II蛋白存在于体细胞组织中，尤其是这些卵泡的卵母细胞中。由此可见，激活素A在体外刺激大鼠腔前卵泡生长，促进腔形成。此外，由于激活素A和ActR II是在腔前卵泡内合成的，因此卵泡内激活素可能在早期卵泡发育中起重要作用。
Abstract Preantral follicles (140–160 μm) were isolated mechanically from the ovaries of 10-day-old rats and cultured in groups of four to six for 6 days in medium containing 0, 1, 10, or 100 ng/ml of activin A. Activin stimulated (P < 0.05) the growth of preantral follicles in a dose-dependent fashion and enhanced the proliferation of preantral, oocyte-free follicular cells. Furthermore, treatment with activin induced the majority of follicles to form an antrum-like structure and helped to maintain the ultrastructure of these follicles during culture. Activin A also induced further changes characteristic of follicle and oocyte maturation, such as the elongation of granulosa cells contacting the oocyte and migration of the cortical granules to the oocyte cortex. In addition, gene expression for activin and activin receptor type II (ActR II) was demonstrated in both the oocytes and the somatic cells using the reverse transcription-polymerase chain reaction, and immunohistochemical studies demonstrated the presence of activin and ActR II proteins in the somatic tissue and, especially, the oocytes of these follicles. It is concluded that, in vitro, activin A stimulates the growth of rat preantral follicles and promotes antrum formation. Furthermore, because activin A and ActR II are synthesized within preantral follicles, intrafollicular activin likely plays an important role in early follicular development.