Modulation of inflammatory response by cortisol in the kidney of spotted scat (Scatophagus argus) in vitro under different osmotic stresses
Modulation of inflammatory response by cortisol in the kidney of spotted scat (Scatophagus argus) in vitro under different osmotic stresses
复制标题
不同渗透压下体外皮质醇对斑鱼（Scatophagus argus）肾脏炎症反应的调节作用
DOI：
10.1016/j.fsi.2020.05.060
复制
发表时间：
2020-09-01
影响因子：
4.7
通讯作者：
Zhang, Junbin
中科院分区：
文献类型：
--
作者：
Su, Maoliang;Zhang, Ran;Zhang, Junbin
盐度变化对肾脏免疫调节的影响已被广泛研究，而渗透胁迫下肾脏的免疫反应在硬骨鱼类中还知之甚少。急性应激通常与循环皮质醇的增强有关。渗透应激对肾脏免疫反应的影响以及皮质醇对其的调节值得更多关注。在本研究中，外源性皮质醇处理对脂多糖（LPS）诱导的免疫反应的影响进行了分析在不同的渗透胁迫下的乌鳢肾肿块在体外。在短期（15 h）内测量促炎细胞因子（TNF-α、IL 1-β和IL-6）和免疫调节相关基因（GR和SOCS 1）的mRNA表达。综合分析表明，由LPS单独或与皮质醇组合诱导的促炎细胞因子基因（如TNF-α、IL-1 β和IL-6）的转录丰度与急性渗透应激下的TNF-α调节密切相关。我们的研究结果表明，渗透压挑战可以显着提高促炎细胞因子的mRNA表达水平在体外肾肿块。基于我们的分析，可以推断，皮质醇抑制肾脏炎症反应的幅度，并通过GR信号转导减弱LPS诱导的免疫反应，在面对挑战性的环境条件。
Salinity changes on renal osmoregulation have often been investigated while the immune response of the kidney under osmotic stress is poorly understood in teleosts. Acute stress is generally associated with enhancement of circulating cortisol. The effects of osmotic stress on renal immune response and its regulation by cortisol deserve more attention. In the present study, the effects of exogenous cortisol treatment on the lipopolysaccharide (LPS)-induced immune response were analyzed in renal masses of Scatophagus argus under different osmotic stresses in vitro. mRNA expression of pro-inflammatory cytokines (TNF-alpha, IL1-beta and IL-6) and immune-regulatory related genes (GR and SOCS1) was measured over a short course (15 h). Comprehensive analysis reveals that transcript abundances of pro-inflammatory cytokine genes such as TNF-alpha, IL-1 beta, and IL-6 induced by LPS, alone or in the combination of cortisol, are tightly associated with osmoregulation under acute osmotic stress. Our results showed that osmotic challenge could significantly enhance mRNA expression levels of pro-inflammatory cytokines in renal masses in vitro. Based on our analysis, it can be inferred that cortisol suppresses the magnitude of renal inflammatory response and attenuates LPS-induced immune response through GR signaling in the face of challenging environmental conditions.