Imaging fast neural traffic at fascicular eve with electrical impedance tomography: proof of principle in rat sciatic nerve
Imaging fast neural traffic at fascicular eve with electrical impedance tomography: proof of principle in rat sciatic nerve
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DOI：
10.1088/1741-2552/aad78e
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发表时间：
2018-10-01
影响因子：
4
通讯作者：
Holder, David
中科院分区：
文献类型：
--
作者：
Aristovich, Kirill;Donega, Matteo;Holder, David
客观的。了解神经束中神经活动的编码是计算神经科学、电疗法自主神经刺激和用于治疗截瘫的功能性电刺激的首要任务。不幸的是，由于尚无技术可以对周围神经束内的神经元去极化进行成像，因此对其进行的研究很少。方法。我们报告了一种实现这一目标的新方法。使用放置在神经周围的柔性圆柱形多电极袖带和快速神经电阻抗断层扫描 (EIT) 的新型医学成像技术。在大鼠坐骨神经中，可以区分响应胫后神经和腓总神经的直接电刺激而激活的单独神经束。主要结果。重建的束激活 EIT 图像与组织学中明显的相应束以及复合动作电位 (CAP) 的逆源分析 (ISA) 具有高空间精度。通过该方法，实现了0.3 ms的时间分辨率和小于100 μm的空间分辨率。意义。这里提出的方法是具有高空间精度的周围神经内成像活动的潜在解决方案。它还为将成像和选择性神经调节纳入单个可植入非穿透性神经周围装置中提供了基础。
Objective. Understanding the coding of neural activity in nerve fascicles is a high priority in computational neuroscience, electroceutical autonomic nerve stimulation and functional electrical stimulation for treatment of paraplegia. Unfortunately, it has been little studied as no technique has yet been available to permit imaging of neuronal depolarization within fascicles in peripheral nerve. Approach. We report a novel method for achieving this. using a flexible cylindrical multi-electrode cuff placed around nerve and the new medical imaging technique of fast neural electrical impedance tomography (EIT). In the rat sciatic nerve, it was possible to distinguish separate fascicles activated in response to direct electrical stimulation of the posterior tibial and common peroneal nerves. Main results. Reconstructed EIT images of fascicular activation corresponded with high spatial accuracy to the appropriate fascicles apparent in histology, as well as the inverse source analysis (ISA) of compound action potentials (CAP). With this method, a temporal resolution of 0.3 ms and spatial resolution of less than 100 mu m was achieved. Significance. The method presented here is a potential solution for imaging activity within peripheral nerves with high spatial accuracy. It also provides a basis for imaging and selective neuromodulation to be incorporated in a single implantable nonpenetrating peri-neural device.