Intracerebroventricular injection of adiponectin regulates locomotor activity in rats
Intracerebroventricular injection of adiponectin regulates locomotor activity in rats
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DOI：
10.2152/jmi.62.199
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发表时间：
2015-08-01
影响因子：
0.7
通讯作者：
Sakaue, Hiroshi
中科院分区：
文献类型：
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作者：
Miyatake, Yumiko;Shiuchi, Tetsuya;Sakaue, Hiroshi
增强运动动机是预防肥胖和糖尿病的最好方法。在这项研究中，我们使用两种类型的行为分析方法来研究脂联素是否影响Wister和自发跑步的德岛-四国(运动)大鼠的运动活动：家庭笼子和车轮跑步活动。运动大鼠是从我们实验室表现出高水平车轮运动活动的Wister品系的原始品系建立的。侧脑室注射脂联素使Wister大鼠和运动大鼠家笼活动量减少，但对摄食量和耗氧量无明显影响。这一结果表明，脂联素可能通过中枢神经系统减少非运动活动产热(NEAT)和体力活动。相比之下，注射脂联素并没有改变运动大鼠的车轮跑步活动。我们用谷氨酸单钠(MSG)制作大鼠下丘脑损伤模型，以阐明脂联素的调节部位。注射脂联素对味精运动大鼠的家笼活动量和摄食量没有影响，提示脂联素对家笼活动的作用在下丘脑区。这些结果提示，脂联素通过下丘脑内侧基底区调节运动活动。
Enhancing exercise motivation is the best way to prevent obesity and diabetes. In this study, we examined whether adiponectin affects locomotion activity in Wister and Spontaneously-Running Tokushima-Shikoku (SPORTS) rats using two types of behavioral assays : home cage and wheel running activity. SPORTS rats were established from an original line fromWister strain that had shown high level of wheel running activity in our laboratory. Injection of adiponectin into the lateral ventricle of Wister rats and SPORTS rats decreased home cage activity, but no change was observed in the food intake and oxygen consumption. This result indicates the possibility that adiponectin can reduce non-exercise activity thermogenesis (NEAT) and physical activity via the central nervous system. In contrast, injection of adiponectin did not change wheel running activity in SPORTS rats. We produced hypothalamus-destructed model rat using monosodium glutamate (MSG) to elucidate the regulation site of adiponectin. Injection of adiponectin into MSG-treated SPORTS rats did not change amount of home cage activity and food intake, suggesting that adiponectin action on home cage activity was in the hypothalamic area. These results suggest that adiponectin regulates locomotion activity through mediobasal hypothalamus.