Immune defense is the primary function associated with the differentially expressed genes in the cochlea following acoustic trauma.
Immune defense is the primary function associated with the differentially expressed genes in the cochlea following acoustic trauma.
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DOI：
10.1016/j.heares.2015.10.010
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发表时间：
2016-03
期刊：
影响因子：
2.8
通讯作者：
Hu BH
中科院分区：
文献类型：
--
作者：
Yang S;Cai Q;Vethanayagam RR;Wang J;Yang W;Hu BH
我们之前对大鼠耳蜗基因进行的 RNA 测序分析确定了耳蜗对声音过度刺激的反应中的多种生物过程和分子途径。然而，其他物种共有的生物过程和分子途径尚未被记录。识别这些常见的应力过程对于更好地理解耳蜗对声损伤的基本反应至关重要。在这里，我们比较了从遭受相似但不相同的声损伤的小鼠和大鼠身上收集的 RNA 测序数据。耳蜗基因的转录组分析鉴定了小鼠和大鼠样本中的差异表达基因。生物信息学分析揭示了两个物种之间生物过程变化的显着相似性，尽管差异表达的基因没有很好地重叠。与差异表达基因相关的常见过程主要与免疫和炎症相关，包括免疫反应、受伤反应、防御反应、趋化性和炎症反应。此外，分子途径的分析显示这两个物种之间有相当大的重叠。常见的信号通路包括细胞因子-细胞因子受体相互作用、趋化因子信号通路、Toll样受体信号通路和NOD样受体信号通路。对转录调控因子的进一步分析揭示了差异表达基因的常见上游调控因子，并且这些上游调控因子也与免疫和炎症反应在功能上相关。这些结果表明，免疫和炎症反应是耳蜗对声音过度刺激的基本反应。
Our previous RNA-sequencing analysis of the rat cochlear genes identified multiple biological processes and molecular pathways in the cochlear response to acoustic overstimulation. However, the biological processes and molecular pathways that are common to other species have not been documented. The identification of these common stress processes is pivotal for a better understanding of the essential response of the cochlea to acoustic injury. Here, we compared the RNA-sequencing data collected from mice and rats that sustained a similar, but not identical, acoustic injury. The transcriptome analysis of cochlear genes identified the differentially expressed genes in the mouse and rat samples. Bioinformatics analysis revealed a marked similarity in the changes in the biological processes between the two species, although the differentially expressed genes did not overlap well. The common processes associated with the differentially expressed genes are primarily associated with immunity and inflammation, which include the immune response, response to wounding, the defense response, chemotaxis and inflammatory responses. Moreover, analysis of the molecular pathways showed considerable overlap between the two species. The common pathways include cytokine-cytokine receptor interactions, the chemokine signaling pathway, the Toll-like receptor signaling pathway, and the NOD-like receptor signaling pathway. Further analysis of the transcriptional regulators revealed common upstream regulators of the differentially expressed genes, and these upstream regulators are also functionally related to the immune and inflammatory responses. These results suggest that the immune and inflammatory responses are the essential responses to acoustic overstimulation in the cochlea.