Localization of gamma-glutamyl transferase activity and protein in Zea mays organs and tissues
Localization of gamma-glutamyl transferase activity and protein in Zea mays organs and tissues
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DOI：
10.1016/j.jplph.2006.07.016
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发表时间：
2007-11-01
影响因子：
4.3
通讯作者：
Ferretti, Massimo
中科院分区：
文献类型：
--
作者：
Masi, Antonio;Destro, Tiziana;Ferretti, Massimo
γ-谷氨酰转移酶/转肽酶（GGT，（5-L-谷氨酰）-肽：氨基酸5-谷氨酰转移酶; EC 2.3.2.2.）是一种促进谷胱甘肽（GSH）和γ-谷氨酰相关化合物γ-谷氨酰部分裂解的胞外酶。在这项工作中，我们描述了GGT的酶组织化学定位。和免疫组织化学。玉米植株的分析。我们的研究结果表明，GGT活性的组织空间分布与GGT蛋白的定位密切相关。GGT活性和蛋白质在组织中分布不均匀，在表皮和气孔、传导元件薄壁组织和根分生组织中较高。这些结果有助于我们了解GGT的功能和调节，以及它在谷胱甘肽代谢中的作用。迄今为止，这些在植物中基本上是未知的。(C)2006年Elsevier GmbH。All rights reserved.
Gamma-glutamyl transferase/transpeptidase (GGT, (5-L-glutamyl)-peptide: aminoacid 5-glutamyltransferase; EC 2.3.2.2.) is an ectoenzyme promoting the cleavage of the gamma-glutamyl moiety of glutathione (GSH) and gamma-glutamyl related compounds. In this work, we describe the localization of GGT by enzymehistochemical. and immunohistochemical. analysis in maize plants. Our results show that the tissue spatial distribution of GGT activity closely correlates with the localization of the GGT protein. We also demonstrate that GGT activity and protein are unevenly distributed in tissues, being higher in the epidermis and stomata, parenchyma of conductive elements and root meristem. These results can contribute to our understanding of GGT function and regulation as well as its role in glutathione metabolism. To date, these are largely unknown in plants. (C) 2006 Elsevier GmbH. All rights reserved.