Peptide characterization of secretin preparations.
Peptide characterization of secretin preparations.
复制标题
促胰液素制剂的肽表征。
DOI：
10.1007/bf01318116
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
McGuigan,JE
中科院分区：
文献类型：
--
作者：
Wolfe,MM;Howard,JE;Kolts,BE;McGuigan,JE
本研究旨在检查和比较GIH和靴子分泌素制剂的肽组成和相对免疫化学纯度。胃肠道肽测定采用放射免疫法使用抗体促胰液素，胃泌素，免疫反应性胆囊收缩素，血管活性肠肽，胃抑制肽，生长抑素。靴子分泌素被发现含有大量的胃泌素，免疫反应性胆囊收缩素，血管活性肠肽，胃抑制肽，和生长抑素。相反，GIH分泌素只含有非常少量的血管活性肠肽。每单位胃肠道分泌液中的分泌素含量大约是Boots分泌素的三到四倍。静脉输注靴子，但不是GIH，分泌素在7名健康志愿者产生显着增加静脉血浆中的所有肽。这些研究的结果表明，Boots促胰液素含有大量和不同量的促胰液素以外的胃肠道肽，并且不同批次的促胰液素和其他肽的含量不同。由于这些肽的量足以显著增加其血液水平和随之而来的生物学效应，因此得出结论，在疑似慢性胰腺炎或胃泌素瘤患者的临床评价中，GIH优于Boots分泌素。
The present study was designed to examine and compare the peptide composition and relative immunochemical purity of GIH and boots secretin preparations. Gastrointestinal peptides were measured by radioimmunoassay using antibodies to secretin, gastrin, immunoreactive cholecystokinin, vasoactive intestinal peptide, gastric inhibitory peptide, and somatostatin. Boots secretin was found to contain substantial quantities of gastrin, immunoreactive cholecystokinin, vasoactive intestinal peptide, gastric inhibitory peptide, and somatostatin. In contrast, GIH secretin contained only a very small amount of vasoactive intestinal peptide. GIH also contained approximately three to four times more secretin per unit as did Boots secretin. Intravenous infusion of Boots, but not GIH, secretin in seven healthy volunteers produced significant increases in venous plasma of all peptides. Results of these studies indicate that Boots secretin contains large and variable quantities of gastrointestinal peptides other than secretin and that the contents of both secretin and the other peptides vary among different lots. Because the quantity of these peptides is sufficient to increase significantly their blood levels and consequent biological effects, it is concluded that GIH is preferable to Boots secretin in the clinical evaluation of patients with suspected chronic pancreatitis or gastrinoma.