A monomeric StayGold fluorescent protein.

A monomeric StayGold fluorescent protein.
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单体 StayGold 荧光蛋白。

DOI:
10.1038/s41587-023-02018-w
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发表时间:
2023
影响因子:
46.9
通讯作者:
Ivorra-Molla E
Ivorra-Molla E
中科院分区:
工程技术1区
文献类型:
--
作者:
Ivorra-Molla E

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StayGold是一种非常明亮和稳定的荧光蛋白,高度抵抗光漂白。尽管具有良好的荧光特性,但StayGold作为荧光标记的使用受到限制,因为它形成了一种天然的二聚体。在这里,我们报告了StayGold的1.6 ä结构,并产生了一种衍生物,mStayGold,它保留了原始蛋白质的亮度和光稳定性,同时又是完全单体的。
StayGold is an exceptionally bright and stable fluorescent protein that is highly resistant to photobleaching. Despite favorable fluorescence properties, use of StayGold as a fluorescent tag is limited because it forms a natural dimer. Here we report the 1.6 Å structure of StayGold and generate a derivative, mStayGold, that retains the brightness and photostability of the original protein while being fully monomeric.
DOI: 10.1002/prot.10286
发表时间: 2003-02-15
期刊: PROTEINS-STRUCTURE FUNCTION AND GENETICS
影响因子: --
作者:
Lovell, SC;Davis, IW;Richardson, DC
通讯作者: Richardson, DC
DOI: 10.1016/j.str.2017.12.006
发表时间: 2018-02-06
期刊: STRUCTURE
影响因子: 5.7
作者:
Campbell, Benjamin C.;Petsko, Gregory A.;Liu, Ce Feng
通讯作者: Liu, Ce Feng