Expression of microRNAs, miR-21, miR-31, miR-122, miR-145, miR-146a, miR-200c, miR-221, miR-222, and miR-223 in patients with hepatocellular carcinoma or intrahepatic cholangiocarcinoma and its prognostic significance
Expression of microRNAs, miR-21, miR-31, miR-122, miR-145, miR-146a, miR-200c, miR-221, miR-222, and miR-223 in patients with hepatocellular carcinoma or intrahepatic cholangiocarcinoma and its prognostic significance
复制标题
DOI：
10.1002/mc.21864
复制
发表时间：
2013-04-01
影响因子：
4.6
通讯作者：
Voros, Dionysios
中科院分区：
文献类型：
--
作者：
Karakatsanis, Andreas;Papaconstantinou, Ioannis;Voros, Dionysios
MicroRNA是一类非编码分子，被发现在健康和疾病中调节各种细胞功能。microRNA的失调参与肝脏疾病，特别是肝癌的发生。由于原发性肝脏恶性肿瘤的典型特征是诊断晚、复发频繁和对辅助治疗的反应差，因此需要发现新的生物标志物以实现早期诊断、预测肿瘤侵袭性和对辅助治疗的反应。本研究的目的是评估某些microRNA（miR-21、-31、-122、-145、-146 a、-200 c、-221、-222和-223）在肝细胞癌（HCC）和肝内胆管癌（ICC）患者中的表达，并评估其预后意义。通过逆转录和实时PCR（RT-PCR）评估Micro-RNA表达。检索并分析临床病理资料和生存率。结果显示，miR-21、miR-31、miR-122、miR-221、miR-222在HCC组织中表达上调，而miR-145、miR-146 a、miR-200 c、miR-223在HCC组织中表达下调。对于ICC样品，发现miR-21、miR-31和miR-223过表达，而miR-122、miR-145、miR-200 c、miR-221和miR-222下调。此外，miR-21、miR-31、miR-122和miR-221在HCC中的表达与肝硬化相关，而miR-21和miR-221与肿瘤分期和不良预后相关。在ICC组织中，发现miR-21、miR-31和miR-223过表达，但未发现与临床病理学特征的相关性。
MicroRNAs are a class of non-coding molecules found to regulate a variety of cellular functions in health and disease. Dysregulation of microRNAs is involved in liver disease, especially hepatocarcinogenesis. Since primary hepatic malignancies are typically characterized by late diagnosis, frequent recurrence, and poor response to adjuvant therapy, there is a need for the discovery of novel biomarkers in order to achieve earlier diagnosis, predict tumor aggressiveness and response to adjuvant therapy. The purpose of this study is to evaluate the expression of certain microRNAs (miR-21, -31, -122, -145, -146a, - 200c, -221, -222 and -223) in patients with hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC), as well as to assess their prognostic significance. Micro-RNA expression was assessed by reverse transcription and real-time PCR (RT-PCR). Clinicopathological data and survival rates were retrieved and analyzed. According to our results, miR-21, miR-31, miR-122, miR-221, miR-222 were significantly up-regulated in HCC tissues, whereas miR-145, miR-146a, miR-200c, and miR-223 were found to be down-regulated. Concerning ICC samples, miR-21, miR-31, and miR-223 were found to be over-expressed, whereas miR-122, miR-145, miR-200c, miR-221, and miR-222 were down-regulated. Additionally, expression of miR-21, miR-31, miR-122, and miR-221 in HCC correlated with cirrhosis, while miR-21 and miR-221 associated with tumor stage and poor prognosis. In ICC tissues, miR-21, miR-31, and miR-223 were found to be over-expressed, but no correlation with clinicopathological features was found. (c) 2011 Wiley Periodicals, Inc.