Selenium Deficiency Mainly Influences the Gene Expressions of Antioxidative Selenoproteins in Chicken Muscles
Selenium Deficiency Mainly Influences the Gene Expressions of Antioxidative Selenoproteins in Chicken Muscles
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缺硒主要影响鸡肌肉抗氧化硒蛋白基因表达
DOI：
10.1007/s12011-014-0125-2
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发表时间：
2014-12-01
影响因子：
3.9
通讯作者：
Xu, Shiwen
中科院分区：
文献类型：
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作者：
Yao, Haidong;Zhao, Wenchao;Xu, Shiwen
日粮缺硒可导致鸡肌肉营养不良，但其分子机制尚不清楚。本研究旨在探讨膳食缺硒对25种硒蛋白表达的影响。1日龄肉鸡饲喂低硒日粮(0.033 mg Se/kg，产于黑龙江缺硒地区中国)和补硒日粮(亚硒酸钠0.20 mg/kg)，共55天。然后，检测了鸡肌肉中25种硒蛋白的mRNA水平，并对其主成分进行了进一步分析。结果表明，抗氧化硒蛋白尤其是Gpxs和Sepw1在鸡肌肉中的表达高于其他类型的硒蛋白。在2 5种硒蛋白中，Gpxs、Txnrd2、TxnRd3、Dio1、Dio3、SELK、SELES、SEW1、SELH、SELU、SELB、SEPT1、SELO、SEX1和SPS2下调(P<； 0.0 5)，其他硒蛋白不受影响(P>； 0.0 5)。缺硒降低了19个硒蛋白的表达(P<； 0.05)，其中11个为抗氧化性硒蛋白。主成分分析进一步表明，抗氧化硒蛋白，特别是Gpx3、Gpx4和Sepw1，可能在鸡肉中发挥重要作用。然而，与这些抗氧化性硒蛋白相比，其他一些低表达的硒蛋白(Dio1、Selu、Selpb、Sepp1)在缺Se的情况下过度降低(超过60%，P<； 0.05)。因此，它可能会节省有限的硒水平，并有利于保持一些关键的硒蛋白的水平。这些结果表明，缺硒主要影响鸡肌肉中抗氧化硒蛋白的表达。而且，抗氧化的硒蛋白，特别是Gpxs和Sepw1可能在鸡肉中起着关键作用。因此，这有助于我们在研究硒在鸡肌肉中的作用时，专注于一些特定的硒蛋白。
Dietary selenium (Se) deficiency induces muscular dystrophy in chicken, but the molecular mechanism remains unclear. The aim of the present study was to investigate the effect of dietary Se deficiency on the expressions of 25 selenoproteins. One-day-old broiler chickens were fed either an Se deficiency diet (0.033 mg Se/kg; produced in the Se-deficient area of Heilongjiang, China) or a diet supplemented with Se (as sodium selenite) at 0.2 mg/kg for 55 days. Then, the mRNA levels of 25 selenoproteins in chicken muscles were examined, and the principal component was further analyzed. The results showed that antioxidative selenoproteins especially Gpxs and Sepw1 were highly and extensively expressed than other types of selenoproteins in chicken muscles. In 25 selenoproteins, Gpxs, Txnrd2, Txnrd 3, Dio1, Dio 3, Selk, Sels, Sepw1, Selh, Sep15, Selu, Selpb, Sepp1, Selo, Sepx1, and SPS2 were downregulated (P< 0.05), and other selenoproteins were not influenced (P> 0.05). Se deficiency decreased the expressions of 19 selenoproteins (P< 0.05), 11 of which were antioxidative selenoproteins. And, principal component analysis (PCA) further indicated that antioxidative selenoproteins, especially Gpx3, Gpx4, and Sepw1, may play crucial roles in chicken muscles. However, compared with these antioxidative selenoproteins, some other lower expressed selenoproteins (Dio1, Selu, Selpb, Sepp1) were excessively decreased (more than 60 %,P< 0.05) by Se deficiency. Thus, it may save the limited Se levels and be beneficial to remain the level of some crucial selenoproteins. These results suggested that Se deficiency mainly influenced the expressions of antioxidative selenoproteins in chicken muscles. And, antioxidative selenoproteins especially Gpxs and Sepw1 may play a crucial role in chicken muscles. Thus, it helps us focus on some specific selenoproteins when studying the role of Se in chicken muscles.