Taurine increases testicular function in aged rats by inhibiting oxidative stress and apoptosis
Taurine increases testicular function in aged rats by inhibiting oxidative stress and apoptosis
复制标题
牛磺酸通过抑制氧化应激和细胞凋亡来增强老年大鼠的睾丸功能
DOI：
10.1007/s00726-015-1995-0
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发表时间：
2015-08-01
期刊：
影响因子：
3.5
通讯作者：
Hu, Jianmin
中科院分区：
文献类型：
--
作者：
Yang, Jiancheng;Zong, Xiaomeng;Hu, Jianmin
在男性中，雄激素合成、精子发生和性功能的下降是衰老的主要表型，这可能归因于睾丸功能障碍。牛磺酸可作为抗氧化剂、睾酮分泌刺激剂、精子膜稳定剂和运动因子以及抗凋亡剂。最近的观察研究表明，牛磺酸可能在精子发生中发挥重要作用，但迄今为止，牛磺酸是否对睾丸具有抗衰老作用仍不清楚。结果发现，牛磺酸可显著提高老龄大鼠睾丸SDH和G6 PDH活性、睾丸标志酶活性、血清睾酮水平、睾丸3 β-HSD和17 β-HSD mRNA表达水平。牛磺酸还能显著提高精子数、活率和活动率，降低精子畸形率。提示牛磺酸可延缓老年大鼠睾丸功能的衰退。牛磺酸可显著提高老龄大鼠睾丸抗氧化酶（SOD、GSH、GSH-Px）活性，降低ROS和MDA含量，提示牛磺酸可降低老龄大鼠睾丸氧化应激和脂质过氧化反应。牛磺酸能有效降低衰老大鼠睾丸DNA片段化，增加睾丸Bcl-2蛋白表达，降低细胞色素c、Bax、Fas、FasL和caspase-3蛋白表达，提示牛磺酸可通过线粒体依赖和非依赖信号通路抑制衰老大鼠睾丸细胞凋亡。总之，我们的研究结果表明，牛磺酸可以通过增加抗氧化能力和抑制细胞凋亡来抑制睾丸功能恶化。
In males, the decline of androgen synthesis, spermatogenesis and sexual function are the main phenotypes of aging, which may be attributed to testicular dysfunction. Taurine can act as an antioxidant, a testosterone secretion stimulator, a sperm membrane stabilizer and motility factor, and an anti-apoptotic agent. Recent observational studies suggested that taurine may play an important role in spermatogenesis, but to date whether taurine has anti-aging effects on testes remains unknown. We found that in aged rats testicular SDH and G6PDH activities, marker enzymes of testes, serum testosterone, testicular 3 beta-HSD and 17 beta-HSD mRNA expression levels were significantly increased by taurine treatment. Taurine administration also markedly raised the sperm count, viability and motility, decreased the sperm abnormality. Our data suggested that taurine can postpone testicular function deterioration in aged rats. Importantly, we observed obvious elevation of testicular antioxidant enzymes (SOD, GSH, GSH-Px) activities, and remarkable reduction of ROS and MDA by taurine administration, indicating taurine can decrease testicular oxidative stress and lipid peroxidation in aged rats. Finally, we found taurine effectively reduced testicular DNA fragmentation, increased testicular Bcl-2 protein expression, and decreased cytochrome c, Bax, Fas, FasL and caspase-3 expression, suggesting taurine can prohibit aged testicular apoptosis by mitochondrial dependent and independent signal pathway. In summary, our results indicated that taurine can suppress testicular function deterioration by increasing antioxidant ability and inhibiting apoptosis.